Taniguchi Kaori, Funasaki Mariko, Kishida Akio, Sadhu Samir K, Ahmed Firoj, Ishibashi Masami, Ohsaki Ayumi
Institute of Biomaterials and Bioengineering, Tokyo Medicinal and Dental University, Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
College of Humanities and Sciences, Nihon University, Setagaya-ku, Tokyo 156-8550, Japan.
Bioorg Med Chem Lett. 2018 Apr 1;28(6):1063-1066. doi: 10.1016/j.bmcl.2018.02.022. Epub 2018 Feb 12.
Two new coumarins (1, 2) and a new xanthone (3), together with 14 known compounds-eight coumarins (4, 5, 9, 10, 12-15), three xanthones (11, 16, 17), a benzoic acid (6) and two flavonones (7, 8)-were isolated from the leaves of Rhizophora mucronata. The structures of the compounds were elucidated by spectroscopic (IR, MS, and NMR) analyses. The isolated compounds were tested for cytotoxicity against human cancer cell lines HL-60 and HeLa. Among these compounds, only compound 16 inhibited the growth of both HeLa (IC = 4.8 μM) and HL-60 (IC = 1.0 μM) cells. Compounds 4, 7, 10, and 12 exhibited moderate activity against HeLa cells (IC = 3.8-8.3 μM). Compounds 5, 9, 11, and 17 showed moderate activity against HL-60 cells (IC = 2.2-6.3 μM). Higher selectivity against HL-60 cell lines was observed for compounds 5, 9, 11, and 16 with SI values (NIH 3T3/HL-60) of 8.6, 19.2, 9.4, and 10.2, respectively.
从红树（Rhizophora mucronata）的叶子中分离出两种新的香豆素（1, 2）和一种新的呫吨酮（3），以及14种已知化合物——8种香豆素（4, 5, 9, 10, 12 - 15）、3种呫吨酮（11, 16, 17）、一种苯甲酸（6）和两种黄酮酮（7, 8）。通过光谱分析（红外光谱、质谱和核磁共振）确定了这些化合物的结构。对分离出的化合物进行了针对人癌细胞系HL - 60和HeLa的细胞毒性测试。在这些化合物中，只有化合物16抑制了HeLa（IC = 4.8 μM）和HL - 60（IC = 1.0 μM）细胞的生长。化合物4、7、10和12对HeLa细胞表现出中等活性（IC = 3.8 - 8.3 μM）。化合物5、9、11和17对HL - 60细胞表现出中等活性（IC = 2.2 - 6.3 μM）。化合物5、9、11和16对HL - 60细胞系具有更高的选择性，其SI值（NIH 3T3/HL - 60）分别为8.6、19.2、9.4和10.2。
