Döring G, Goldstein W, Botzenhart K, Kharazmi A, Schiøtz P O, Høiby N, Dasgupta M
Clin Exp Immunol. 1986 Jun;64(3):597-605.
In sputa of 21 cystic fibrosis patients suffering from chronic Pseudomonas aeruginosa lung infections, activities of lysosomal proteases released from polymorphonuclear leukocytes (PMN) and immune complexes were quantitatively determined. The results showed that low immune complex values correlated with high protease activities and vice versa. In a longitudinal study fluctuating, reciprocal changes of the parameters were observed. Therefore, the hypothesis was investigated as to whether PMN elastase cleaves immune complexes and regulates inflammation. It was shown that treatment of immune complex-positive sputa with PMN elastase decreased IgG and IgA immune complex values and lead to cleavage of the immunoglobulins in the hinge region. PMN elastase-treated samples were not able to stimulate the oxidative burst of PMN. Cleavage of in vitro built immune complexes did not lead to liberation of bound antigen. Western blot analysis of one sputum sample revealed split products of the IgG heavy chain. The results suggest that PMN elastase splits off the Fc portion of immunoglobulins in immune complexes and thus regulates inflammation by a feedback mechanism leading to cyclic inflammatory states.
在21例患有慢性铜绿假单胞菌肺部感染的囊性纤维化患者的痰液中，对多形核白细胞（PMN）释放的溶酶体蛋白酶活性和免疫复合物进行了定量测定。结果表明，低免疫复合物值与高蛋白酶活性相关，反之亦然。在一项纵向研究中，观察到参数的波动和相互变化。因此，研究了PMN弹性蛋白酶是否裂解免疫复合物并调节炎症的假设。结果表明，用PMN弹性蛋白酶处理免疫复合物阳性痰液可降低IgG和IgA免疫复合物值，并导致免疫球蛋白在铰链区裂解。PMN弹性蛋白酶处理的样品不能刺激PMN的氧化爆发。体外构建的免疫复合物的裂解不会导致结合抗原的释放。对一份痰液样本进行的蛋白质印迹分析显示了IgG重链的裂解产物。结果表明，PMN弹性蛋白酶裂解免疫复合物中免疫球蛋白的Fc部分，从而通过导致周期性炎症状态的反馈机制调节炎症。
