新型多聚磷酸葡萄糖激酶、肌醇 1-磷酸合酶和肌醇单磷酸酶三酶级联反应从葡萄糖生产肌醇。
Production of myo-inositol from glucose by a novel trienzymatic cascade of polyphosphate glucokinase, inositol 1-phosphate synthase and inositol monophosphatase.
机构信息
School of Life Science and Medicine, Dalian University of Technology, Dalian, China; Institute of Microbiology, Chinese Academy of Sciences, CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Beijing, China.
Institute of Microbiology, Chinese Academy of Sciences, CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Beijing, China.
出版信息
Enzyme Microb Technol. 2018 May;112:1-5. doi: 10.1016/j.enzmictec.2018.01.006. Epub 2018 Jan 31.
Myo-inositol (inositol) is important in the cosmetics, pharmaceutical and functional food industries. Here, we report a novel pathway to produce inositol from glucose by a trienzymatic cascade system involving polyphosphate glucokinase (PPGK), inositol 1-phosphate synthase (IPS) and inositol monophosphatase (IMP). The system contained three highly active enzymes, AspPPGK from Arthrobacter sp. OY3WO11, TbIPS from Trypanosoma brucei TREU927, and EcIMP from Escherichia coli. A trienzymatic cascade reaction was implemented, and the conversion ratio from glucose to inositol reached 90%, which is promising for the enzymatic synthesis of inositol without ATP supplementation.
肌醇（inositol）在化妆品、制药和功能性食品行业中很重要。在这里，我们报告了一种从葡萄糖生产肌醇的新途径，该途径涉及多聚磷酸葡萄糖激酶（PPGK）、肌醇 1-磷酸合酶（IPS）和肌醇单磷酸酶（IMP）的三酶级联系统。该系统包含三种高活性酶，来自节杆菌属 OY3WO11 的 AspPPGK、来自布氏锥虫 TREU927 的 TbIPS 和来自大肠杆菌的 EcIMP。实施了三酶级联反应，葡萄糖到肌醇的转化率达到 90%，这为无需补充 ATP 的肌醇酶促合成提供了广阔的前景。
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