Khandy M T, Titova M V, Konstantinova S V, Kochkin D V, Ivanov I M, Nosov A M
Prikl Biokhim Mikrobiol. 2016 Nov-Dec;52(6):614-20.
Changes in the content of the furostanol glycosides protodioscin and deltoside, particularly that of the (25S)-isomers of the glycosides, during suspension cultivation of different lines of Nepal yam (Dioscorea deltoidea Wall.) cells of the strain IFR-DM-0.5 has been investigated. The composition of furostanol glycosides has been characterized, and the dynamics of the accumulation of individual glycosides during lengthy subcultivation of cells maintained in flasks or in a barbotage bioreactor has been analyzed. A positive correlation between the growth and accumulation of substances that belonged to the class of furostanol glycosides has been demonstrated for cultured dioscorea cells, whereas the content of some of the individual glycosides varied considerably between the lines of the strain, cultures maintained under different conditions, and even between cells in different phases of the growth cycle. The increased content of (25R)-forms of the glycosides (protodioscin and deltoside) was correlated with a decrease in the cellular growth rate, whereas an increase in culture growth intensity occurred concomitantly to an increase of the amount of (25S)-isomers. This may be indicative of the specific stimulatory effect of (25S)-glycosides, but not the (25R)-forms, on cell proliferation in vitro. Thus, the concentration of (25S)-forms may increase due to the autoselection of cells capable of intensive division during prolonged cultivation.
对尼泊尔薯蓣（Dioscorea deltoidea Wall.）IFR-DM-0.5菌株不同细胞系悬浮培养过程中呋甾烷醇糖苷原薯蓣皂苷和三角叶皂苷含量的变化进行了研究，尤其关注了糖苷（25S）-异构体的含量变化。对呋甾烷醇糖苷的组成进行了表征，并分析了在烧瓶或鼓泡式生物反应器中连续传代培养的细胞中各糖苷积累的动态变化。已证明培养的薯蓣细胞中属于呋甾烷醇糖苷类的物质的生长与积累之间存在正相关，然而，某些单个糖苷的含量在该菌株的不同细胞系、不同培养条件下甚至在生长周期不同阶段的细胞之间存在很大差异。糖苷（原薯蓣皂苷和三角叶皂苷）（25R）-形式含量的增加与细胞生长速率的降低相关，而培养生长强度的增加与（25S）-异构体含量的增加同时发生。这可能表明（25S）-糖苷而非（25R）-形式对体外细胞增殖具有特定的刺激作用。因此，在长时间培养过程中，由于能够进行密集分裂的细胞的自动选择，（25S）-形式的浓度可能会增加。
