Agarwal Astha, Yadava Pranjal, Kumar Krishan, Singh Ishwar, Kaul Tanushri, Pattanayak Arunava, Agrawal Pawan Kumar
1Indian Council of Agricultural Research- Indian Institute of Maize Research, Pusa Campus, New Delhi, 110012 India.
2Department of Biology, Stanford University, 385 Serra Mall, Stanford, CA 94305 USA.
Physiol Mol Biol Plants. 2018 Mar;24(2):175-183. doi: 10.1007/s12298-017-0502-3. Epub 2018 Feb 17.
Maize is an important crop for billions of people as food, feed, and industrial raw material. It is a prime driver of the global agricultural economy as well as the livelihoods of millions of farmers. Genetic interventions, such as breeding, hybridization and transgenesis have led to increased productivity of this crop in the last 100 years. The technique of genome editing is the latest advancement in genetics. Genome editing can be used for targeted deletions, additions, and corrections in the genome, all aimed at genetic enhancement of crops. The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated protein 9 (CRISPR/Cas9) system is a recent genome editing technique that is considered simple, precise, robust and the most revolutionary. This review summarizes the current state of the art and predicts future directions in the use of the CRISPR/Cas9 tool in maize crop improvement.
玉米是数十亿人的重要作物，用作食物、饲料和工业原料。它是全球农业经济以及数百万农民生计的主要驱动力。在过去100年里，诸如育种、杂交和转基因等遗传干预措施提高了这种作物的产量。基因组编辑技术是遗传学领域的最新进展。基因组编辑可用于基因组的靶向缺失、添加和校正，所有这些都是为了提高作物的遗传特性。成簇规律间隔短回文重复序列（CRISPR）/CRISPR相关蛋白9（CRISPR/Cas9）系统是一种最新的基因组编辑技术，被认为简单、精确、强大且最具革命性。本文综述了CRISPR/Cas9工具在玉米作物改良中的应用现状，并预测了未来的发展方向。
