Department of Pharmacology, UFR-SMA, Félix Houphouët-Boigny University, 01 BP V 166 Abidjan 01, Abidjan, Côte d'Ivoire.
UMR CNRS 7213, Laboratory of Biophotonics and Pharmacology, Faculty of Pharmacy, University of Strasbourg, Illkirch, France.
BMC Complement Altern Med. 2018 Mar 15;18(1):94. doi: 10.1186/s12906-018-2158-0.
Phyllanthus amarus (Schum & Thonn), a plant belonging to the family of Euphorbiaceae is used in Ivorian traditional medicine to treat cardiovascular disorders such as hypertension. However, although this plant has been described as a diuretic agent, the underlying mechanism remains unclear. Therefore, the aim of the present study was to investigate the mechanism action of diuretic effects of an ethanolic fraction of Phyllanthus amarus (EFPA) in rats.
Effects of EFPA on urinary excretion were carried out for doses ranging from 5 to 80 mg/kg given by intraperitoneal injection (i.p.) and compared with that induced by furosemide (5 mg/kg) after 8 h. Thereafter, the diuretic activity of EFPA was also evaluated in the presence of indomethacin (5 mg/kg, i.p.) in order to determine the involvement of prostaglandins, after 24 h.
Between 5 and 80 mg/kg, EFPA induced a significant urinary excretion. The profile of urinary excretion showed that after 2 h, the highest dose of 80 mg/kg induced a urinary volumetric excretion (UVE), which was similar to that induced by furosemide. After 24 h, EFPA at 10 mg/kg increased significantly UVE, Na (43 mEq) and Cl (97 mEq) urinary excretions without promoting kaliuresis. In rats pretreated with indomethacin, the urinary excretion and the natriuretic response of EFPA were significantly reduced.
Altogether, this study has shown that EFPA promotes a significant urinary excretion of water and Na, confirming its diuretic activity. Moreover, the increased diuresis could be attributed, at least in part, to the involvement of prostaglandins.
叶下珠（Schum & Thonn）是大戟科植物，在科特迪瓦传统医学中用于治疗心血管疾病，如高血压。然而，尽管这种植物已被描述为利尿剂，但作用机制尚不清楚。因此，本研究旨在探讨叶下珠的乙醇提取物（EFPA）对大鼠利尿作用的机制。
EFPA 以 5 至 80mg/kg 的剂量经腹腔注射给药，评估其对尿排泄的影响，并与呋塞米（5mg/kg）的作用进行比较，在 8 小时后观察。然后，在 24 小时后，通过腹腔注射吲哚美辛（5mg/kg）来评估 EFPA 的利尿活性，以确定前列腺素的参与情况。
在 5 至 80mg/kg 剂量范围内，EFPA 诱导了显著的尿排泄。尿排泄模式显示，在 2 小时后，最高剂量 80mg/kg 诱导了与呋塞米相似的尿容量排泄（UVE）。在 24 小时后，EFPA 以 10mg/kg 的剂量显著增加 UVE、Na（43mEq）和 Cl（97mEq）的尿排泄，但没有促进尿钾排泄。在预先用吲哚美辛处理的大鼠中，EFPA 的尿排泄和排钠反应明显减少。
总的来说，本研究表明 EFPA 促进了水和 Na 的显著尿排泄，证实了其利尿活性。此外，利尿作用的增强至少部分归因于前列腺素的参与。
