Kong Hang-Kin, Wong Ka-Hing, Lo Samuel Chun-Lap
Food Safety and Technology Research Center, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong; Shenzhen Key Laboratory for Food Biological Safety Control, PolyU Shenzhen Research Institute, Shenzhen, China.
Food Safety and Technology Research Center, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong; Shenzhen Key Laboratory for Food Biological Safety Control, PolyU Shenzhen Research Institute, Shenzhen, China.
Food Res Int. 2016 Jul;85:19-25. doi: 10.1016/j.foodres.2016.04.002. Epub 2016 Apr 16.
For centuries, edible bird's nest (EBN) has been consumed as a Chinese delicacy. In the past decades, numerous studies reported that water soluble extract of the EBN not only possessed epidermal growth factor, but also associated with a wide range of health-promoting effects. However, based on the traditional Chinese way of EBN preparation and consumption, the bioactive components should be originated from both its hot water soluble and insoluble fractions. Nevertheless, information on the hot water insoluble fraction (HWIF) of EBN is not currently available. In this study, peptides released from the HWIF of EBN under simulated gastro-intestinal conditions were identified for the first time by de novo sequencing using a combination of MALDI TOF/TOF MS and ESI-ion-trap MS/MS. The released peptides were found to share very high similarities to mucin, NADH dehydrogenase, acidic mammalian chitinase-like protein, immunoglobulin, proline-rich protein, von Willebrand factor and epidermal growth factor domain-containing protein.
几个世纪以来，食用燕窝一直是一种中国美食。在过去几十年里，大量研究报告称，燕窝的水溶性提取物不仅含有表皮生长因子，还具有广泛的促进健康的作用。然而，根据中国传统的燕窝制备和食用方式，其生物活性成分应该同时来源于其热水溶性和不溶性部分。然而，目前尚无关于燕窝热水不溶性部分（HWIF）的信息。在本研究中，首次通过结合基质辅助激光解吸电离飞行时间串联质谱（MALDI TOF/TOF MS）和电喷雾离子阱串联质谱（ESI-ion-trap MS/MS）的从头测序，鉴定了在模拟胃肠道条件下从燕窝HWIF中释放的肽。发现释放的肽与粘蛋白、NADH脱氢酶、酸性哺乳动物几丁质酶样蛋白、免疫球蛋白、富含脯氨酸的蛋白、血管性血友病因子和含表皮生长因子结构域的蛋白具有非常高的相似性。
