Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, PR, Brazil.
Departamento de Farmacologia, Universidade Federal do Paraná, CP 19.046, CEP 81.531-980, Curitiba, PR, Brazil.
Int J Biol Macromol. 2018 Oct 15;118(Pt B):1414-1421. doi: 10.1016/j.ijbiomac.2018.06.164. Epub 2018 Jul 2.
Jambo is a tropical fruit cultivated in Southeast Asia and tropical regions of America and Africa. After extraction, its polysaccharides were structurally characterized. Water soluble polysaccharides (WSP) were composed of GalA:Ara:Gal:Glc:Rha in 51:22:16:5:6 molar ratio, indicating the presence of pectic polysaccharides. Methylation analysis and NMR spectroscopy indicated the presence of homogalacturonan (HG), type II arabinogalactan and type I rhamnogalacturonan (RG-I). The HG/RG-I ratio was 88%, indicating greater amounts of smooth than hairy pectic regions. Hemicellulosic polysaccharides were extracted from the residue and fractionated by freeze-thaw procedure in two fractions (ASP-S and ASP-I). ASP-S was composed of Glc:Gal:Xyl:Ara:Man:Fuc:UA in a 45:16:20:3:8:5:2 molar ratio. Methylation analysis and C NMR spectroscopy indicated xyloglucan, xylan and mannan. Their relative proportion estimated on sugar linkage was 89%, 6% and 3%, respectively. ASP-I was composed mainly of xylose (99.5%) and its C NMR indicated a linear (1 → 4)-β-D-xylan. The biological activity of WSP was tested in Ehrlich tumor-bearing mice. After 21 days of oral treatment, the doses of 150 and 250 mg/kg WSP reduced expressively the tumor growth, similarly to the positive control methotrexate, and improved the body weight of tumor-bearing mice. Further studies are necessary to investigate the mechanisms of the WSP antitumor effect.
布氏罗望子是一种热带水果，生长在东南亚和美洲、非洲的热带地区。提取后，对其多糖进行了结构表征。水溶性多糖（WSP）由 GalA:Ara:Gal:Glc:Rha 以 51:22:16:5:6 的摩尔比组成，表明存在果胶多糖。甲基化分析和 NMR 光谱表明存在同质半乳糖醛酸聚糖（HG）、Ⅱ型阿拉伯半乳聚糖和Ⅰ型鼠李半乳糖醛酸聚糖（RG-I）。HG/RG-I 比为 88%，表明平滑果胶区域比绒毛状果胶区域更多。从残渣中提取半纤维素多糖，并通过冷冻-解冻程序在两个馏分（ASP-S 和 ASP-I）中进行分级。ASP-S 由 Glc:Gal:Xyl:Ara:Man:Fuc:UA 以 45:16:20:3:8:5:2 的摩尔比组成。甲基化分析和 C NMR 光谱表明存在木葡聚糖、木聚糖和甘露聚糖。根据糖键估计它们的相对比例分别为 89%、6%和 3%。ASP-I 主要由木糖（99.5%）组成，其 C NMR 表明存在线性（1 → 4）-β-D-木聚糖。在荷瘤小鼠中测试了 WSP 的生物活性。经口治疗 21 天后，150 和 250 mg/kg WSP 剂量可显著抑制肿瘤生长，与阳性对照甲氨蝶呤相似，并改善荷瘤小鼠的体重。需要进一步研究以探讨 WSP 抗肿瘤作用的机制。
