Forbes-Haley Claire, Jones Siân Bodfel, Davies Maria, West Nicola X
UHBristol, Bristol Dental Hospital, Lower Maudlin Street, Bristol BS1 2LY, UK.
Clinical Trials Unit, School of Oral and Dental Sciences, University of Bristol, Lower Maudlin Street, Bristol BS1 2LY, UK.
Dent J (Basel). 2016 Aug 9;4(3):25. doi: 10.3390/dj4030025.
To develop an in vitro model to mimic the effects of meals equivalent to a day's diet on tooth tissue loss (TTL). To identify how diet effects tooth wear and to test the efficacy of dental products designed to reduce tooth wear in a more realistic environment. A typical Friday diet was devised comprising: Breakfast then brushing, lunch, dinner then brushing. Groups of enamel samples were exposed to one meal, or all three in series, a control group was exposed to water and brushed. The daily cycle was repeated to represent two days' consumption; TTL was quantified by non-contact profilometry. This pilot study highlighted adaptions that could be made to the model such as human enamel and saliva to further replicate natural eating habits. The sum of the TTL measured after Breakfast, lunch and dinner (bovine enamel specimens exposed to single meals) was less than that exhibited by the group of samples exposed to the series of meals but this difference was not significant ( = 0.09).In the absence and presence of brushing, TTL caused by breakfast and dinner was similar, but significantly greater than that caused by lunch ( < 0.05). While brushing increased TTL, this increase was not significant. It is possible to model a daily diet in vitro, and the data obtained confirms that the combination of food and drink affects the degree of TTL. This supports the further development of an in vitro model that includes alternative foodstuffs. This would aid understanding of the effects different diets have on TTL and could test new products designed to prevent TTL.
建立一个体外模型，以模拟相当于一天饮食的餐食对牙齿组织损耗（TTL）的影响。确定饮食如何影响牙齿磨损，并在更现实的环境中测试旨在减少牙齿磨损的牙科产品的功效。设计了一种典型的周五饮食：早餐后刷牙，午餐，晚餐后刷牙。将几组牙釉质样本分别暴露于一餐或连续三餐，对照组暴露于水中并刷牙。重复每日循环以代表两天的摄入量；通过非接触式轮廓测量法定量TTL。这项初步研究突出了可以对模型进行的调整，例如使用人类牙釉质和唾液，以进一步复制自然饮食习惯。早餐、午餐和晚餐后测量的TTL总和（暴露于单餐的牛牙釉质标本）小于暴露于三餐系列的样本组，但这种差异不显著（P = 0.09）。在有无刷牙的情况下，早餐和晚餐引起的TTL相似，但显著大于午餐引起的TTL（P < 0.05）。虽然刷牙会增加TTL，但这种增加并不显著。可以在体外模拟日常饮食，获得的数据证实食物和饮料的组合会影响TTL的程度。这支持进一步开发包含替代食物的体外模型。这将有助于理解不同饮食对TTL的影响，并可以测试旨在预防TTL的新产品。
