Natural Products Research Laboratories, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK.
Phytother Res. 2010 Aug;24(8):1181-7. doi: 10.1002/ptr.3096.
The need to discover and develop alternative therapies to treat methicillin-resistant Staphylococcus aureus (MRSA) infections is timely. This study was undertaken to purify and identify some anti-MRSA constituents from propolis, a natural product from the beehive traditionally used in folk medicine for its antimicrobial properties. A crude extract of propolis originating from the Solomon Islands ('Pacific propolis') was screened, using an agar dilution assay, in vitro against 15 MRSA clinical isolates. Results revealed activity worthy of further investigation, and subsequent purification work on this crude extract afforded 23 fractions. Further purification of active fractions led to the isolation of compounds 1-4, characterized upon analysis of their spectroscopic data (1D- and 2D-NMR, MS) and by comparison with the literature, as the prenylflavanones propolin H (1), propolin G (2), propolin D (3), and propolin C (4). This study is the first to report the anti-MRSA activity of 'Pacific propolis' and the presence of prenylflavanones in the propolis sample selected. The anti-MRSA activity of propolin D (3) (MIC 8-16 mg/L) and propolin C (4) (MIC 8-32 mg/L) is reported for the first time.
发现和开发替代疗法来治疗耐甲氧西林金黄色葡萄球菌（MRSA）感染的需求是及时的。本研究旨在从蜂胶中分离和鉴定一些抗 MRSA 的成分，蜂胶是一种来自蜂巢的天然产物，传统上用于民间医学，具有抗菌特性。用琼脂稀释法对源自所罗门群岛的粗提蜂胶（“太平洋蜂胶”）进行了筛选，以测试其对 15 株 MRSA 临床分离株的体外抗菌活性。结果表明，这种粗提物具有进一步研究的价值，随后对该粗提物进行了进一步的分离纯化，得到了 23 个馏分。对活性馏分的进一步纯化导致分离出化合物 1-4，通过分析其光谱数据（1D 和 2D-NMR、MS）并与文献进行比较，确定为烯丙基黄酮醇 propolin H（1）、propolin G（2）、propolin D（3）和 propolin C（4）。本研究首次报道了“太平洋蜂胶”的抗 MRSA 活性和所选蜂胶样品中存在烯丙基黄酮醇。首次报道了 propolin D（3）（MIC 8-16 mg/L）和 propolin C（4）（MIC 8-32 mg/L）的抗 MRSA 活性。
