Oliveira Ana M P, Devesa Joana S P, Hill Peter B
Faculty of Veterinary Medicine, University Lusófona de Humanidades e Tecnologias, Campo Grande 376, 1749-024, Lisbon, Portugal.
School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, South Australia, 5371, Australia.
Vet Dermatol. 2018 Jun;29(3):180-e65. doi: 10.1111/vde.12533. Epub 2018 Mar 22.
Staphylococcus pseudintermedius and Malassezia pachydermatis are important agents in canine pyoderma and otitis.
HYPOTHESIS/OBJECTIVES: Determine the in vitro efficacy of a honey-based gel (HBO) against meticillin-susceptible S. pseudintermedius (MSSP), meticillin-resistant S. pseudintermedius (MRSP) and M. pachydermatis, by minimum bactericidal concentration (MBC), minimum fungicidal concentration (MFC) and time-kill assay (TKA). Efficacy of the product's honey component (HO) also was evaluated.
Sixty S. pseudintermedius and 10 M. pachydermatis canine isolates were selected. All isolates were tested against serial dilutions of an HBO containing 40% HO (40%, 20%, 10%, 5% and 2.5% w/v) and HO alone (undiluted, 40%, 20%, 10%, 5% and 2.5% w/v). Microbroth assay followed by subculture was used to determine MBC and MFC. The same protocol was applied after product exposure to catalase. A well-diffusion assay for S. pseudintermedius was used to generate inhibition zones. A TKA for 10 isolates of S. pseudintermedius and 10 isolates of M. pachydermatis was performed.
MBC was 20% w/v (5-20% w/v) for HBO and HO. HBO had lower MBC values when compared to HO (P = 0.003). No statistical difference was observed between MSSP/MRSP isolates (HBO P = 0.757, HO P = 0.743). Only HO was affected by catalase (P = 0.015). MFC for HBO was 10% w/v (5-10% w/v) and 40% w/v for HO (20-≥40% w/v). All isolates were killed after 4 h of exposure.
Staphylococcus pseudintermedius and M. pachydermatis are susceptible to the HBO and these results can be used for future clinical trials.
中间型假单胞菌和厚皮马拉色菌是犬脓皮病和中耳炎的重要病原体。
假设/目标：通过最低杀菌浓度（MBC）、最低杀真菌浓度（MFC）和时间 - 杀菌试验（TKA），确定一种蜂蜜基凝胶（HBO）对甲氧西林敏感的中间型假单胞菌（MSSP）、甲氧西林耐药的中间型假单胞菌（MRSP）和厚皮马拉色菌的体外疗效。还评估了该产品蜂蜜成分（HO）的疗效。
选择60株犬源中间型假单胞菌和10株厚皮马拉色菌分离株。所有分离株均针对含40% HO的HBO系列稀释液（40%、20%、10%、5%和2.5% w/v）以及单独的HO（未稀释、40%、20%、10%、5%和2.5% w/v）进行测试。采用微量肉汤法继以传代培养来确定MBC和MFC。产品经过氧化氢酶处理后应用相同方案。对中间型假单胞菌采用平板扩散法生成抑菌圈。对10株中间型假单胞菌分离株和10株厚皮马拉色菌分离株进行TKA。
HBO和HO的MBC均为20% w/v（5 - 20% w/v）。与HO相比，HBO的MBC值更低（P = 0.003）。MSSP/MRSP分离株之间未观察到统计学差异（HBO，P = 0.757；HO，P = 0.743）。只有HO受过氧化氢酶影响（P = 0.015）。HBO的MFC为10% w/v（5 - 10% w/v），HO的MFC为40% w/v（20 - ≥40% w/v）。所有分离株暴露4小时后均被杀死。
中间型假单胞菌和厚皮马拉色菌对HBO敏感，这些结果可用于未来的临床试验。
