Department of Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, 90095, USA.
Department of Bioengineering, University of California, Los Angeles, CA, 90095, USA.
Curr Cardiol Rep. 2018 Mar 24;20(5):35. doi: 10.1007/s11886-018-0979-6.
Real-time 3-dimensional (3-D) imaging of cardiovascular injury and regeneration remains challenging. We introduced a multi-scale imaging strategy that uses light-sheet illumination to enable applications of cardiovascular injury and repair in models ranging from zebrafish to rodent hearts.
Light-sheet imaging enables rapid data acquisition with high spatiotemporal resolution and with minimal photo-bleaching or photo-toxicity. We demonstrated the capacity of this novel light-sheet approach for scanning a region of interest with specific fluorescence contrast, thereby providing axial and temporal resolution at the cellular level without stitching image columns or pivoting illumination beams during one-time imaging. This cutting-edge imaging technique allows for elucidating the differentiation of stem cells in cardiac regeneration, providing an entry point to discover novel micro-circulation phenomenon with clinical significance for injury and repair. These findings demonstrate the multi-scale applications of this novel light-sheet imaging strategy to advance research in cardiovascular development and regeneration.
目的综述：心血管损伤和再生的实时三维（3-D）成像是一个具有挑战性的问题。我们提出了一种多尺度成像策略，该策略使用光片照明，可在从斑马鱼到啮齿动物心脏的模型中应用心血管损伤和修复。
最新发现：光片成像能够以高时空分辨率进行快速数据采集，同时具有最小的光漂白或光毒性。我们证明了这种新型光片方法能够对具有特定荧光对比度的感兴趣区域进行扫描，从而在单次成像过程中无需拼接图像列或旋转照明光束即可在细胞水平上提供轴向和时间分辨率。这种尖端的成像技术可用于阐明心脏再生中干细胞的分化，为发现具有损伤和修复临床意义的新微循环现象提供切入点。这些发现表明，这种新型光片成像策略具有多尺度应用，可推进心血管发育和再生研究。
