Helmholtz-Centre for Environmental Research GmbH-UFZ; Department of Soil System Science, Halle (Saale), Germany.
Martin-Luther-University Halle-Wittenberg; Institute of Agricultural and Nutritional Sciences, Halle (Saale), Germany.
PLoS One. 2018 Mar 26;13(3):e0193669. doi: 10.1371/journal.pone.0193669. eCollection 2018.
X-ray CT is a powerful technology to study root growth in soil in-situ. Root systems can be studied in its true 3D geometry over time. Hence, the same plant can be scanned multiple times during development. A downside is the potential of X-rays to interfere with biological processes and therefore plant growth. The aim of this study is to evaluate the influence of cumulative X-ray dose on Vicia faba and Hordeum vulgare during a growth period of 17 days. One control treatment without X-ray scanning was compared to two treatments being scanned every two and four days, respectively. Scanned treatments received a maximum cumulative dose of less than 8 Gy. Plant species differed in their susceptibility to X-ray dose. For Vicia faba, mean total root length was reduced significantly. Leave growth was reduced as well. Number and length of second order laterals was reduced significantly, as well as length of first order laterals. Hordeum vulgare showed no negative impact of X-ray dose on any of the root parameters. Large differences between the two species investigated were detected in respect to susceptibility to X-ray dose. Results indicate that for X-ray CT studies involving temporal resolution a control treatment without scanning is required.
X 射线 CT 是一种强大的技术，可用于原位研究土壤中的根系生长。根系可以随时间以真实的 3D 几何形状进行研究。因此，同一植物可以在发育过程中多次扫描。缺点是 X 射线可能会干扰生物过程并因此影响植物生长。本研究的目的是评估在 17 天的生长期间，累积 X 射线剂量对 Vicia faba 和 Hordeum vulgare 的影响。将不进行 X 射线扫描的对照处理与每两天和四天扫描一次的两种处理进行比较。扫描处理的累积剂量最高不超过 8Gy。植物物种对 X 射线剂量的敏感性不同。对于 Vicia faba，总根长的平均值显著降低。叶片生长也减少了。第二级侧根的数量和长度以及第一级侧根的长度也显著减少。Hordeum vulgare 显示 X 射线剂量对任何根系参数均无负面影响。在对 X 射线剂量的敏感性方面，研究的两个物种之间存在很大差异。结果表明，对于涉及时间分辨率的 X 射线 CT 研究，需要进行不进行扫描的对照处理。
