Hassaan Mayar H, Rashad Nagah A, El Sawa Afaf A, Eldesouky Lubna M, Sedik Aya S
Department of Oral Biology, College of Dentistry, Arab Academy for Science, Technology & Maritime Transport, El Alamein, Egypt.
Department of Oral Biology, Faculty of Dentistry, Alexandria University, Alexandria, Egypt.
BMC Oral Health. 2025 Jun 21;25(1):949. doi: 10.1186/s12903-025-06299-2.
Dentin hypersensitivity (DH) is a prevalent dental issue characterized by sharp pain of short duration. Although the exact cause of DH remains debated, the hydrodynamic theory explains its mechanism. Furthermore, various methods, such as potassium-containing toothpaste, mouthwash, and chewing gum, have been attempted to address DH. However, recent research has explored the potential of nanoparticles (NPs) for DH treatment due to their biocompatibility and bioactive properties.
Twenty-seven sound premolars extracted for orthodontic purposes were utilized in the present study to obtain 27 mid-coronal dentin discs. These discs were then etched with 37% orthophosphoric acid for 20 s to expose dentinal tubules (DTs) and simulate DH. Subsequently, dentin discs were rinsed with distilled water for 1 min. These dentin discs were randomly allocated into 3 groups: Group I (etched control), Group II (the etched dentin discs were treated with a carboxymethyl cellulose (CMC) dental hydrogel scaffold loaded with 20% HANPs), and Group III (the etched dentin discs were treated with a CMC dental hydrogel scaffold loaded with 20% ZnO NPs). After 7 days, Group II and III underwent erosive challenge to evaluate the protective effect of the nanoparticle treatments. Finally, the dentin discs were analyzed using profilometric analysis to measure surface roughness (SRa), scanning electron microscopy (SEM) to evaluate DT occlusion, computer-assisted SEM image analysis using Image J.
Among the groups, Group I exhibited the highest SRa following the DH simulation. Group II showed lower SRa compared to group III. SEM analysis indicated that Group III displayed more occluded DTs compared to Group II. Group I showed the most unoccluded DTs. The SEM analysis results were further quantitatively confirmed using Image J.
The results indicated that both HANPs and ZnO NPs exhibited promising outcomes; however, ZnO NPs demonstrated superior effectiveness in resisting erosive wear compared to HANPs.
牙本质过敏症（DH）是一种常见的牙科问题，其特征为短时间的尖锐疼痛。尽管DH的确切病因仍存在争议，但流体动力学说解释了其发病机制。此外，人们尝试了各种方法来解决DH问题，如含钾牙膏、漱口水和口香糖。然而，由于纳米颗粒（NPs）具有生物相容性和生物活性特性，最近的研究探索了其在治疗DH方面的潜力。
本研究使用了27颗因正畸目的而拔除的健康前磨牙，以获取27个牙冠中部牙本质盘。然后用37%的正磷酸对这些牙本质盘蚀刻20秒，以暴露牙本质小管（DTs）并模拟DH。随后，用蒸馏水冲洗牙本质盘1分钟。这些牙本质盘被随机分为3组：第一组（蚀刻对照组），第二组（用负载20%羟基磷灰石纳米颗粒（HANPs）的羧甲基纤维素（CMC）牙科水凝胶支架处理蚀刻后的牙本质盘），第三组（用负载20%氧化锌纳米颗粒（ZnO NPs）的CMC牙科水凝胶支架处理蚀刻后的牙本质盘）。7天后，对第二组和第三组进行侵蚀性挑战，以评估纳米颗粒处理的保护效果。最后，使用轮廓仪分析测量表面粗糙度（SRa），用扫描电子显微镜（SEM）评估DT闭塞情况，使用Image J进行计算机辅助SEM图像分析，对牙本质盘进行分析。
在各组中，第一组在模拟DH后表现出最高的SRa。与第三组相比，第二组的SRa较低。SEM分析表明，与第二组相比，第三组显示出更多闭塞的DTs。第一组显示出最未闭塞的DTs。使用Image J进一步定量证实了SEM分析结果。
结果表明，HANPs和ZnO NPs均显示出有前景的结果；然而，与HANPs相比，ZnO NPs在抵抗侵蚀性磨损方面表现出更优异的效果。
