Adhisivam B, Vishnu Bhat B, Rao Krishna, Kingsley S M, Plakkal Nishad, Palanivel C
a Department of Neonatology , Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER) , Pondicherry , India.
J Matern Fetal Neonatal Med. 2019 Sep;32(18):3016-3019. doi: 10.1080/14767058.2018.1455089. Epub 2018 Mar 27.
The objective of this study was to study the effect of Holder pasteurization on macronutrients and immunoglobulin profile of pooled donor human milk. This descriptive study was conducted in a Human Milk Bank of a tertiary care teaching institute in south India. Thirty random paired pooled donor human milk samples (before and after pasteurization) were analyzed for macronutrients (protein, fat, carbohydrates) using infrared spectroscopy. Similarly, immunoglobulin profile (IgA and IgG) before and after pasteurization was quantified using ELISA. The mean values of protein, fat, and carbohydrates in pooled donor milk pre-pasteurization were 1.6, 3.6, and 6.1 g/dl compared with post-pasteurization values 1.4, 2.7, and 5.9 g/dl, respectively. Pasteurization reduced protein, fat, and energy content of pooled donor milk by 12.5%, 25%, and 16%, respectively. However, carbohydrates were not significantly reduced. Pasteurization decreased IgA by 30% and IgG by 60%. Holder pasteurization of pooled donor human milk decreases protein, fat, and energy content and also reduces the levels of IgA and IgG.
本研究的目的是探讨低温长时间巴氏杀菌对混合捐赠人乳常量营养素和免疫球蛋白谱的影响。这项描述性研究在印度南部一家三级护理教学机构的母乳库中进行。使用红外光谱法对30份随机配对的混合捐赠人乳样本（巴氏杀菌前后）进行常量营养素（蛋白质、脂肪、碳水化合物）分析。同样，使用酶联免疫吸附测定法对巴氏杀菌前后的免疫球蛋白谱（IgA和IgG）进行定量。混合捐赠人乳巴氏杀菌前蛋白质、脂肪和碳水化合物的平均值分别为1.6、3.6和6.1g/dl，而巴氏杀菌后的值分别为1.4、2.7和5.9g/dl。巴氏杀菌使混合捐赠人乳的蛋白质、脂肪和能量含量分别降低了12.5%、25%和16%。然而，碳水化合物含量没有显著降低。巴氏杀菌使IgA降低了30%，IgG降低了60%。混合捐赠人乳的低温长时间巴氏杀菌会降低蛋白质、脂肪和能量含量，同时也会降低IgA和IgG的水平。
