Institut de Recherche en Sciences de la Santé/Centre Muraz, Bobo-Dioulasso, Burkina Faso.
Laboratoire d'Entomologie Fondamentale et Appliqué/UFR-SVT/Université Ouaga 1, Pr. Joseph KI-Zerbo, Ouagadougou, Burkina Faso.
Parasit Vectors. 2018 Mar 27;11(1):209. doi: 10.1186/s13071-018-2796-6.
Genetically enhanced Metarhizium pingshaense are being developed for malaria vector control in Burkina Faso. However, not much is known about the local prevalence and pathogenicity of this fungus, so we prospected mosquitoes and plant roots (a common habitat for Metarhizium spp.) for entomopathogenic fungi.
Our investigations showed that Metarhizium spp. represented between 29-74% of fungi isolated from plant root rhizospheres in diverse collection sites. At low spore dosages (1 × 10 conidia/ml), two mosquito-derived M. pingshaense isolates (Met_S26 and Met_S10) showed greater virulence against Anopheles coluzzii (LT of ~7 days) than isolates tested in previous studies (LT of ~10 days). In addition, the local isolates did not cause disease in non-target insects (honeybees and cockroaches).
Our work provides promising findings for isolating local Metarhizium strains for application in mosquito biological control and for future transgenic biocontrol strategies in Burkina Faso.
正在为布基纳法索的疟疾病媒控制而开发遗传增强型沙雷氏金龟子虫。然而，对于这种真菌在当地的流行程度和致病性知之甚少，因此我们对蚊子和植物根部（沙雷氏菌属的常见栖息地）进行了病原真菌普查。
我们的调查显示，在不同采集地点的植物根际土壤中，沙雷氏菌属真菌占分离真菌的 29-74%。在低孢子剂量（1×10 个分生孢子/ml）下，两种源自蚊子的沙雷氏金龟子虫分离株（Met_S26 和 Met_S10）对库蚊（LT 约为 7 天）的毒力大于以前研究中测试的分离株（LT 约为 10 天）。此外，当地分离株不会引起非靶标昆虫（蜜蜂和蟑螂）发病。
我们的工作为分离当地沙雷氏菌菌株应用于蚊子生物防治以及未来在布基纳法索开展转基因生物防治策略提供了有希望的发现。
