Department of pharmacy, the Second People's Hospital of Wuxi, Wuxi, P.R. China.
Department of pharmacy, the Forth People's Hospital of Wuxi, Wuxi, P.R. China.
PLoS One. 2018 Mar 28;13(3):e0193449. doi: 10.1371/journal.pone.0193449. eCollection 2018.
Osthole is an active compound isolated from Chinese herb Cnidium monnieri (L.) Cusson, and had been reported to possess antitumor effect. However, the effect of osthole on the gastric cancer cells has not been investigated. In this study, the effects of osthole on the proliferation of human gastric cancer cells were tested. The data showed that osthole treatment significantly inhibited the proliferation of gastric cancer cells and resulted in the cell cycle arrest at G2/M phase in a dose-dependent manner. Western-blot study showed that the expression of cyclin B1 and cdc2 was markedly reduced by osthole. Moreover, expression of PI3K and pAKT was also significantly suppressed, and the results indicated that the inhibition of pAKT, cyclin B1, and cdc2 levels by osthole was notably enhanced by a PI3K inhibitor. These results demonstrate that osthole could inhibit gastric cancer cells proliferation via induction of cell cycle arrest at G2/M phase by the reduction of PI3K/AKT.
蛇床子素是从中国草药蛇床子（Cnidium monnieri（L.）Cusson）中分离得到的一种活性化合物，据报道具有抗肿瘤作用。然而，蛇床子素对胃癌细胞的作用尚未得到研究。在这项研究中，测试了蛇床子素对人胃癌细胞增殖的影响。数据显示，蛇床子素处理显著抑制胃癌细胞的增殖，并呈剂量依赖性导致细胞周期停滞在 G2/M 期。Western-blot 研究表明，蛇床子素明显降低了细胞周期蛋白 B1 和 cdc2 的表达。此外，PI3K 和 pAKT 的表达也明显受到抑制，结果表明，PI3K 抑制剂显著增强了蛇床子素对 pAKT、细胞周期蛋白 B1 和 cdc2 水平的抑制作用。这些结果表明，蛇床子素通过降低 PI3K/AKT 来减少细胞周期蛋白 B1 和 cdc2 的水平，从而诱导 G2/M 期细胞周期停滞，从而抑制胃癌细胞的增殖。
