Computational Medicine Center, Thomas Jefferson University, 1020 Locust Street, Philadelphia, PA, 19107, USA.
Sci Rep. 2018 Mar 28;8(1):5314. doi: 10.1038/s41598-018-22488-2.
MicroRNA (miRNA) isoforms ("isomiRs") and tRNA-derived fragments ("tRFs") are powerful regulatory non-coding RNAs (ncRNAs). In human tissues, both types of molecules are abundant, with expression patterns that depend on a person's race, sex and population origin. Here, we present our analyses of the Prostate Cancer (PRAD) datasets of The Cancer Genome Atlas (TCGA) from the standpoint of isomiRs and tRFs. This study represents the first simultaneous examination of isomiRs and tRFs in a large cohort of PRAD patients. We find that isomiRs and tRFs have extensive correlations with messenger RNAs (mRNAs). These correlations are disrupted in PRAD, which suggests disruptions of the regulatory network in the disease state. Notably, we find that the profiles of isomiRs and tRFs differ in patients belonging to different races. We hope that the presented findings can lay the groundwork for future research efforts aimed at elucidating the functional roles of the numerous and distinct members of these two categories of ncRNAs that are present in PRAD.
微小 RNA（miRNA）异构体（“isomiRs”）和 tRNA 衍生片段（“tRFs”）是强大的调控性非编码 RNA（ncRNA）。在人体组织中，这两种分子都很丰富，其表达模式取决于一个人的种族、性别和种群起源。在这里，我们从 miRNA 和 tRFs 的角度对癌症基因组图谱（TCGA）的前列腺癌（PRAD）数据集进行了分析。这项研究代表了首次在大量 PRAD 患者中同时检查 miRNA 和 tRFs。我们发现 miRNA 和 tRFs 与信使 RNA（mRNA）之间存在广泛的相关性。这些相关性在 PRAD 中被破坏，这表明在疾病状态下调控网络被破坏。值得注意的是，我们发现属于不同种族的患者中 miRNA 和 tRFs 的谱存在差异。我们希望提出的发现能够为未来的研究工作奠定基础，这些研究旨在阐明这两类 ncRNA 中大量不同成员在 PRAD 中的功能作用。
