Department of Chemistry, School of Physical and Mathematical Sciences, University of Kerala, Kariavattom Campus, Thiruvananthapuram, Kerala, 695581, India.
Mikrochim Acta. 2017 Dec 5;185(1):11. doi: 10.1007/s00604-017-2574-8.
Carbon dots doped with sulfur and nitrogen (S,N-CDs) were utilised to design a paper-stripe based fluorescent probe for the detection of bilirubin. The S,N-CDs were synthesized through a microwave assisted route by using citric acid as carbon source and L-cysteine as a source of nitrogen and sulfur. The S,N-CDs exhibit bright blue fluorescence emission with a peak at 452 nm. Fluorescence is quenched by Fe(III) but selectively restored by bilirubin. The quenched fluorescent probe exhibit significant selectivity and sensitivity for bilirubin in the 0.2 nM to 2 nM concentration range, with a 0.12 nM detection limit. The method was applied to the determination of bilirubin in spiked human serum and urine samples. The method was used to design a paper based test stripe as a point of care device for visual bilirubin detection. Graphical abstract Schematic representation of sulphur and nitrogen doped carbon dots whose fluorescence is quenched by Fe(III) and turned on by bilirubin. Photograph of the corresponding system under day light and UV shows the feasibility of the phenomenon. The applicability of the assay was further extended by impregnating the probe on a filter paper.
硫氮共掺杂碳点（S,N-CDs）被用于设计一种基于纸条的荧光探针，用于检测胆红素。S,N-CDs 通过微波辅助法合成，以柠檬酸为碳源，L-半胱氨酸为氮源和硫源。S,N-CDs 发出明亮的蓝色荧光，峰值在 452nm。荧光被 Fe(III) 猝灭，但被胆红素选择性恢复。猝灭的荧光探针对胆红素在 0.2nM 至 2nM 浓度范围内表现出显著的选择性和灵敏度，检测限为 0.12nM。该方法用于测定加标人血清和尿液样品中的胆红素。该方法用于设计基于纸条的测试条，作为即时检测胆红素的床边检测设备。示意图表示硫氮共掺杂的碳点，其荧光被 Fe(III) 猝灭，被胆红素打开。在自然光和紫外光下观察到相应系统的照片，显示了这一现象的可行性。通过将探针浸渍在滤纸上，进一步扩展了该测定方法的适用性。
