Key Laboratory of Textile Science and Technology, Ministry of Education, College of Textiles , Donghua University , Songjiang District , Shanghai 201620 , China.
Modern Textile Institute , Donghua University , Changning District , Shanghai 200051 , China.
ACS Appl Mater Interfaces. 2018 Apr 25;10(16):13304-13316. doi: 10.1021/acsami.7b18927. Epub 2018 Apr 10.
Immediate hemorrhage control and anti-infection play important roles in the wound management. Besides, a moist environment is also beneficial for wound healing. Hydrogels are promising materials in urgent hemostasis and drug release. However, hydrogels have the disadvantage of rapid release profiles, leading to the exposure to high drug concentrations. In this study, we constructed hybrid hydrogels with rapid hemostasis and sustainable antibacterial property combining aminoethyl methacrylate hyaluronic acid (HA-AEMA) and methacrylated methoxy polyethylene glycol (mPEG-MA) hybrid hydrogels and chlorhexidine diacetate (CHX)-loaded nanogels. The CHX-loaded nanogels (CLNs) were prepared by the enzyme degradation of CHX-loaded lysine-based hydrogels. The HA-AEMA and mPEG-MA hybrid hydrogel loaded with CLNs (labeled as Gel@CLN) displayed a three-dimensional microporous structure and exhibited excellent swelling, mechanical property, and low cytotoxicity. The Gel@CLN hydrogel showed a prolonged release period of CHX over 240 h and the antibacterial property over 10 days. The hemostasis and wound-healing properties were evaluated in vivo using a mouse model. The results showed that hydrogel had the rapid hemostasis capacity and accelerated wound healing. In summary, CLN-loaded hydrogels may be excellent candidates as hemostasis and anti-infection materials for the wound dressing application.
立即止血和抗感染在伤口管理中起着重要作用。此外，湿润的环境也有利于伤口愈合。水凝胶是紧急止血和药物释放的有前途的材料。然而，水凝胶具有快速释放曲线的缺点，导致暴露于高药物浓度。在这项研究中，我们构建了具有快速止血和可持续抗菌性能的混合水凝胶，将丙烯酰氨基乙基甲基丙烯酸透明质酸（HA-AEMA）和甲基丙烯酰氧基甲氧基聚乙二醇（mPEG-MA）混合水凝胶与醋酸洗必泰（CHX）负载纳米凝胶结合在一起。CHX 负载的纳米凝胶（CLNs）是通过 CHX 负载的赖氨酸基水凝胶的酶降解制备的。载有 CLN 的 HA-AEMA 和 mPEG-MA 混合水凝胶（标记为 Gel@CLN）显示出三维微孔结构，并表现出优异的溶胀性、机械性能和低细胞毒性。Gel@CLN 水凝胶显示出 CHX 的延长释放期超过 240 h 和超过 10 天的抗菌性能。通过小鼠模型评估了体内止血和伤口愈合性能。结果表明，水凝胶具有快速止血能力并加速伤口愈合。总之，载有 CLN 的水凝胶可能是用作伤口敷料应用的止血和抗感染材料的优秀候选物。
