Cheaib Dina, El Darra Nada, Rajha Hiba N, Maroun Richard G, Louka Nicolas
Faculty of Sciences, Beirut Arab University, Riad El Solh, P.O. Box 115020, Beirut 1107 2809, Lebanon.
Faculty of Health Sciences, Beirut Arab University, Tarik El Jedidah, Riad El Solh, P.O. Box 115020, Beirut 1107 2809, Lebanon.
ScientificWorldJournal. 2018 Jan 29;2018:8249184. doi: 10.1155/2018/8249184. eCollection 2018.
This work aims to study the impact of solvent mixture (between 0 and 50% ethanol/water mixture) and temperature (between 25°C and 75°C) levels on the solid-liquid extraction of phenolic compounds (quantity and bioactivity) from apricot pomace. Results show that the mean augmentation of 1% ethanol in the range [0-12%] enhances by three times the extraction of polyphenols compared to the same augmentation in the range [0-50%]. Similarly, the mean augmentation of 1°Celcius in the range [0-25°Celcius] enhances by two times the extraction of polyphenols compared to the same augmentation in the range [0-75°Celcius]. Moreover, 1% of ethanol exhibited a greater impact on the phenolic compound extraction than 1°Celsius. The response surface methodology showed that the optimal extraction condition was reached with 50% ethanol/water at 75°C giving a total phenolic content (TPC) of 9.8 mg GAE/g DM, a flavonoids content (FC) of 8.9 mg CE/g DM, a tannin content (TC) of 4.72 mg/L, and an antiradical activity (AA) of 44%. High-performance liquid chromatography (HPLC) analysis showed that polyphenols were influenced by the selectivity of the solvent as well as the properties of each phenolic compound. Apricot pomace extracts could therefore be used as natural bioactive molecules for many industrial applications.
本研究旨在探讨溶剂混合物(乙醇/水混合物含量为0至50%)和温度(25°C至75°C)水平对从杏渣中进行固液萃取酚类化合物(数量和生物活性)的影响。结果表明,在[0 - 12%]范围内乙醇含量平均增加1%,与在[0 - 50%]范围内相同增幅相比,多酚的萃取量增加了两倍。同样,在[0 - 25°C]范围内温度平均升高1°C,与在[0 - 75°C]范围内相同增幅相比,多酚的萃取量增加了一倍。此外,1%的乙醇对酚类化合物萃取的影响比1°C更大。响应面法表明,在75°C下使用50%乙醇/水可达到最佳萃取条件,此时总酚含量(TPC)为9.8 mg GAE/g DM,黄酮类化合物含量(FC)为8.9 mg CE/g DM,单宁含量(TC)为4.72 mg/L,抗自由基活性(AA)为44%。高效液相色谱(HPLC)分析表明,多酚受溶剂选择性以及每种酚类化合物性质的影响。因此,杏渣提取物可作为天然生物活性分子用于许多工业应用。