Hu Jiayun, Bohn Paul W
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.
Department of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN 46556, USA.
J Anal Test. 2017 Jan;1(1). doi: 10.1007/s41664-017-0002-z. Epub 2017 Feb 6.
Bacterial sensing is important for understanding the numerous roles bacteria play in nature and in technology, understanding and managing bacterial populations, detecting pathogenic bacterial infections, and preventing the outbreak of illness. Current analytical challenges in bacterial sensing center on the dilemma of rapidly acquiring quantitative information about bacteria with high detection efficiency, sensitivity, and specificity, while operating within a reasonable budget and optimizing the use of ancillary tools, such as multivariate statistics. This review starts from a general description of bacterial sensing methods and challenges, and then focuses on bacterial characterization using optical methods including Raman spectroscopy and imaging, infrared spectroscopy, fluorescence spectroscopy and imaging, and plasmonics, including both extended and localized surface plasmon resonance spectroscopy. The advantages and drawbacks of each method in relation to the others are discussed, as are their applications. A particularly promising direction in bacterial sensing lies in combining multiple approaches to achieve multiplex analysis, and examples where this has been achieved are highlighted.
细菌传感对于理解细菌在自然界和技术中所起的众多作用、理解和管理细菌种群、检测病原菌感染以及预防疾病爆发都很重要。当前细菌传感中的分析挑战集中在这样一个两难困境：即在合理预算范围内运行并优化辅助工具（如多元统计）的使用的同时，要以高检测效率、灵敏度和特异性快速获取有关细菌的定量信息。本综述首先对细菌传感方法和挑战进行一般性描述，然后重点介绍使用光学方法进行细菌表征，包括拉曼光谱和成像、红外光谱、荧光光谱和成像以及等离子体技术，包括扩展和局域表面等离子体共振光谱。讨论了每种方法相对于其他方法的优缺点及其应用。细菌传感中一个特别有前景的方向是结合多种方法以实现多重分析，并突出了已实现这一点的实例。
