Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
Pharmaceutical Sciences Research Center, Ardabil University of Medical Sciences, Ardabil, Iran; Biosensor Sciences and Technologies Research Center Ardabil University of Medical Sciences, Ardabil, Iran.
Talanta. 2025 Jan 1;281:126852. doi: 10.1016/j.talanta.2024.126852. Epub 2024 Sep 16.
Multiplex biosensors are highly sought-after tools in disease diagnosis. This technique involves the simultaneous sensing of multiple biomarkers, whose levels and ratios can provide a more comprehensive assessment of disease conditions compared to single biomarker detection. In most diseases like cancer due to its complexity, several biomarkers are involved in their occurrence. On the other hand, a single biomarker may be implicated in various diseases. Multiplex sensing employs various techniques, such as optical, electrochemical, and electrochemiluminescence methods. This comprehensive review focuses on optical multiplex sensing techniques, including surface plasmon resonance, localized surface plasmon resonance, fluorescence resonance energy transfer, chemiluminescence, surface-enhanced Raman spectroscopy, and photonic crystal sensors. The review delves into their mechanisms, materials utilized, and strategies for biomarker detection.
多重生物传感器是疾病诊断中备受追捧的工具。该技术涉及到对多个生物标志物的同时检测，与单一生物标志物检测相比，其水平和比例可以提供更全面的疾病评估。在大多数疾病中，如癌症，由于其复杂性，涉及到多个生物标志物的参与。另一方面，单一的生物标志物可能与多种疾病有关。多重感应采用了多种技术，如光学、电化学和电致化学发光方法。本综述重点介绍了光学多重感应技术，包括表面等离子体共振、局域表面等离子体共振、荧光共振能量转移、化学发光、表面增强拉曼光谱和光子晶体传感器。该综述深入探讨了它们的机制、所用材料以及生物标志物检测的策略。
