Ko Dong-Jin, Kim Ji-Sun, Park Doo-Sang, Lee Dae-Hee, Heo Sun-Yeon, Seo Jeong-Woo, Kim Chul Ho, Oh Baek-Rock
Microbial Biotechnology Research Center, KRIBB, Jeong-up 56212, Republic of Korea.
Biological Resources Center, KRIBB, Jeong-up 56212, Republic of Korea.
Int J Syst Evol Microbiol. 2018 May;68(5):1800-1805. doi: 10.1099/ijsem.0.002760. Epub 2018 Apr 6.
The translucent white-coloured, Gram-stain-negative, aerobic, non-motile, fusiform-shaped bacterium (designated strain SY72) was isolated from waste-activated sludge. Optimal growth occurred at 30-37 °C and pH 6.0-7.0. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the novel isolate belonged to the family Rhodobacteraceae of the class Alphaproteobacteria. Strain SY72 is closely related to Tabrizicola aquatica KCTC 23724 (97.8 % 16S rRNA gene sequence similarity) and Pseudorhodobacter aquaticus DC2N1-10 (96.4 %), respectively. DNA-DNA relatedness between strain SY72 and the closest phylogenetically related strain, Tabrizicola aquatica KCTC 23724, was 18.0±0.7 %. In strain SY72, the predominant respiratory quinone was ubiquinone Q-10, and the cellular fatty acids consisted mainly of C18 : 1ω7c and C18 : 1ω7c-11 methyl. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol and phosphatidylethanolamine. Photoautotrophic and photoheterotrophic growth did not occur in strain SY72. Furthermore, strain SY72 did not produce photosynthetic pigments or contain the photosynthetic genes pufL and pufM, by which it differed from the phototrophic species of the family Rhodobacteraceae. On the basis of distinct phenotypic and phylogenetic properties, strain SY72 represents a novel species of the genus Tabrizicola, for which the name Tabrizicola fusiformis sp. nov. is proposed. The type strain is SY72 (=KCTC 62105=NBRC 113021).
从活性污泥中分离出一种半透明的白色、革兰氏染色阴性、需氧、不运动、梭形的细菌（命名为菌株SY72）。在30-37°C和pH 6.0-7.0条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，该新分离菌株属于α-变形菌纲红杆菌科。菌株SY72分别与水生塔布里齐菌KCTC 23724（16S rRNA基因序列相似性为97.8%）和水生假红杆菌DC2N1-10（96.4%）密切相关。菌株SY72与系统发育关系最密切的菌株水生塔布里齐菌KCTC 23724之间的DNA-DNA相关性为18.0±0.7%。在菌株SY72中，主要呼吸醌为泛醌Q-10，细胞脂肪酸主要由C18:1ω7c和C18:1ω7c-11甲基组成。主要极性脂为磷脂酰胆碱、磷脂酰甘油、二磷脂酰甘油和磷脂酰乙醇胺。菌株SY72不发生光合自养和光合异养生长。此外，菌株SY72不产生光合色素，也不含有光合基因pufL和pufM，这使其与红杆菌科的光合物种不同。基于明显的表型和系统发育特性，菌株SY72代表塔布里齐菌属的一个新物种，为此提出名为梭形塔布里齐菌（Tabrizicola fusiformis sp. nov.）。模式菌株为SY72（=KCTC 62105=NBRC 113021）。
