Ielciu Irina, Hanganu Daniela, Păltinean Ramona, Vlase Laurian, Frédérich Michel, Gheldiu Ana-Maria, Benedec Daniela, Crişan Gianina
Department of Pharmaceutical Botany, Faculty of Pharmacy, "Iuliu Haţieganu" University of Medicine and Pharmacy, 23 Gheorghe Marinescu Street, Cluj-Napoca, Romania / Laboratory of Pharmacognosy, Center of Interdisciplinary Research on Medicines, University of Liège, 15 Avenue de Hippocrate, B36, Tour 4, Liege, Belgium.
Department of Pharmacognosy, Faculty of Pharmacy, "Iuliu Haţieganu" University of Medicine and Pharmacy,12 Ion Creangă Street, Cluj-Napoca, Romania.
Pak J Pharm Sci. 2018 Mar;31(2(Suppl.)):677-683.
Echinocystis lobata (Michx.) Torr. et A.Gray is a spontaneous species in the Romanian flora, lesser studied by scientific literature, but which has proved significant activities in traditional medicine. The present study is aimed to provide data on the polyphenolic compounds in the composition of the flowers of this species and to test their biological potential. Polyphenols were identified and quantified using an HPLC-MS method. Tested biological activities were the cytotoxic, anti-plasmodial and antioxidant ones. Methods used for testing the antioxidant activity were the DPPH, CUPRAC, FRAP, TEAC, EPR and SNPAC assays. Cytotoxic activity was tested on cancerous and healthy cell lines and anti-plasmodial activity was assesed on two strains of Plasmodium falciparum. Ethanolic extracts of the flowers of E. lobata proved to contain isoquercitrin, rutin, quercitrin, kaempferol, p-coumaric and ferulic acid. No cytotoxic and anti-plasmodial activity was found, but antioxidant assays showed an important antioxidant capacity. The obtained results show that flowers of E. lobata are important sources of antioxidant compounds. It is the first approach of the kind on the flowers of this species and it offers a new perspective on possible sources of antioxidant compounds.
美洲商陆（Echinocystis lobata (Michx.) Torr. et A.Gray）是罗马尼亚植物区系中的一个自生种，科学文献对其研究较少，但已证明它在传统医学中有显著活性。本研究旨在提供该物种花中多酚类化合物的数据，并测试其生物活性。使用高效液相色谱 - 质谱法对多酚进行鉴定和定量。测试的生物活性包括细胞毒性、抗疟和抗氧化活性。用于测试抗氧化活性的方法有DPPH、CUPRAC、FRAP、TEAC、EPR和SNPAC测定法。对癌细胞系和健康细胞系测试细胞毒性活性，并对两种恶性疟原虫菌株评估抗疟活性。美洲商陆花的乙醇提取物被证明含有异槲皮苷、芦丁、槲皮苷、山奈酚、对香豆酸和阿魏酸。未发现细胞毒性和抗疟活性，但抗氧化测定显示出重要的抗氧化能力。所得结果表明，美洲商陆花是抗氧化化合物的重要来源。这是首次对该物种的花进行此类研究，为抗氧化化合物的可能来源提供了新的视角。
