Păltinean Ramona, Ielciu Irina, Hanganu Daniela, Niculae Mihaela, Pall Emoke, Angenot Luc, Tits Monique, Mocan Andrei, Babotă Mihai, Frumuzachi Oleg, Tămaş Mircea, Crişan Gianina, Frederich Michel
Department of Pharmaceutical Botany, "Iuliu Haţieganu" University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania.
Department of Pharmacognosy, "Iuliu Haţieganu" University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania.
Plants (Basel). 2022 Apr 29;11(9):1202. doi: 10.3390/plants11091202.
Soy. Will. is a species belonging to the Papaveraceae family, being widespread in East-Central and Southern Europe. As with numerous other species of the genus, it is used in traditional medicine for the treatment of hepatobiliary and digestive disorders. The aim of the present study consisted of the evaluation of its alkaloid content and the assessment of its in vitro antioxidant, anti-cholinesterase and cytotoxic potential. Total alkaloid content in the composition of the species was quantified by a spectrophotometrical method and they were individually identified and quantified by HPLC-DAD. The antioxidant capacity was investigated by the DPPH and FRAP methods, while the anti-cholinesterase activity was assessed by an adapted Ellman's method. The in vitro cytotoxic activity was evaluated on BJ human fibroblasts and DLD-1 human colon adenocarcinoma cell lines. Results showed the presence of bicuculline, protopine, chelidonine, stylopine and sanguinarine, among which bicuculline, protopine, stylopine and sanguinarine were quantified, while the antioxidant and anti-cholinesterase assays showed valuable potentials. No cytotoxic effect was observed on BJ cell lines and selective cytotoxicity was expressed towards tumoral cells. In this context, appears as an important medicinal species with significant potential of substitution with the officinal species.
紫堇是罂粟科的一种植物，广泛分布于欧洲中东部和南部。与该属的许多其他物种一样，它在传统医学中用于治疗肝胆和消化系统疾病。本研究的目的包括评估其生物碱含量以及评估其体外抗氧化、抗胆碱酯酶和细胞毒性潜力。通过分光光度法对该物种成分中的总生物碱含量进行定量，并通过HPLC-DAD对其进行单独鉴定和定量。通过DPPH和FRAP方法研究抗氧化能力，同时通过改良的Ellman方法评估抗胆碱酯酶活性。在BJ人成纤维细胞和DLD-1人结肠腺癌细胞系上评估体外细胞毒性活性。结果显示存在荷包牡丹碱、原阿片碱、白屈菜碱、斯氏紫堇碱和血根碱，其中对荷包牡丹碱、原阿片碱、斯氏紫堇碱和血根碱进行了定量，而抗氧化和抗胆碱酯酶测定显示出有价值的潜力。在BJ细胞系上未观察到细胞毒性作用，并且对肿瘤细胞表现出选择性细胞毒性。在这种情况下，紫堇似乎是一种重要的药用植物，具有很大的替代药用植物的潜力。
