Department of Crop Science, Faculty of Agriculture, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania.
Department of Pharmaceutical Botany, Faculty of Pharmacy, "Iuliu Haţieganu" University of Medicine and Pharmacy Cluj-Napoca, 400010 Cluj-Napoca, Romania.
Molecules. 2023 Feb 7;28(4):1604. doi: 10.3390/molecules28041604.
The aim of the present study was to correlate the antioxidant, antimicrobial, and cytotoxic activities of hydroalcoholic extracts obtained from the aerial parts of three L. cultivars with their polyphenolic compositions. The polyphenols were identified and quantified using spectrophotometrical methods and LC-MS analysis. Their antioxidant capacities were assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) methods. Their in vitro antimicrobial efficacies were assessed using the agar well diffusion and broth microdilution methods. Their cytotoxicity was investigated on normal diploid foreskin fibroblasts (BJ) and on colorectal adenocarcinoma (DLD-1) cell lines. The results pointed out significant amounts of polyphenolic compounds in the compositions of the tested cultivars, with rosmarinic acid as the main compound (amounts ranging between 5.337 ± 0.0411 and 6.320 ± 0.0535 mg/mL). All three cultivars displayed significant antioxidant (IC ranging between 35.542 ± 0.043 and 40.901 ± 0.161 µg/mL for the DPPH assay, and for the FRAP assay 293.194 ± 0.213 and 330.165 ± 0.754 µmol Trolox equivalent/mg dry vegetal material) and antimicrobial potential (especially towards the Gram-positive bacteria), as well as a selective toxicity towards the tumoral line. A significant positive correlation was found between antioxidant activity and the total phenolic acids ( = 0.987) and polyphenols ( = 0.951). These findings bring further arguments for strongly considering cultivars as promising vegetal products, which warrants further investigation.
本研究旨在探讨三种 品种的地上部分水醇提取物的抗氧化、抗菌和细胞毒性活性与其多酚组成之间的相关性。使用分光光度法和 LC-MS 分析鉴定和定量多酚。使用 1,1-二苯基-2-苦基肼（DPPH）和铁还原抗氧化能力（FRAP）方法评估其抗氧化能力。使用琼脂孔扩散和肉汤微量稀释法评估其体外抗菌功效。在正常二倍体包皮成纤维细胞（BJ）和结直肠腺癌细胞（DLD-1）细胞系上研究其细胞毒性。结果表明，测试品种的组成中含有大量的多酚化合物，以迷迭香酸为主要化合物（含量在 5.337 ± 0.0411 和 6.320 ± 0.0535 mg/mL 之间）。三种品种均表现出显著的抗氧化（DPPH 测定的 IC 范围在 35.542 ± 0.043 和 40.901 ± 0.161 µg/mL 之间，FRAP 测定的 IC 范围在 293.194 ± 0.213 和 330.165 ± 0.754 µmol Trolox 当量/mg 干植物材料）和抗菌潜力（特别是对革兰氏阳性菌），以及对肿瘤系的选择性毒性。抗氧化活性与总酚酸（ = 0.987）和多酚（ = 0.951）之间存在显著正相关。这些发现为强烈考虑 品种作为有前途的植物产品提供了进一步的论据，值得进一步研究。
