Department of Biomedical Engineering, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, Incheon, 21936, Republic of Korea.
Gachon Advanced Institute for Health Science & Technology, Gachon University, Incheon, 21999, Republic of Korea.
Mikrochim Acta. 2018 Apr 7;185(5):250. doi: 10.1007/s00604-018-2770-1.
The authors report on a microneedle-based amperometric nonenzymatic glucose sensor for painless and continuous monitoring of glucose. It consists of 3 × 5 sharp stainless steel microneedles micromachined from a stainless steel substrate. The microneedles are 600 and 100 μm in height and width, respectively. Nafion and platinum black were sequentially coated onto the tip of gold-coated microneedles and used for nonenzymatic (direct) sensing of glucose. Attractive features of the modified microneedle electrode include (a) a low working potential (+0.12 V vs. Ag/AgCl), (b) a linear response in the physiologically relevant range (1-40 mM), (c) a sensitivity as high as 175 μA mM cm, (d) a 23 μM detection limit, and (e) a response time of 2 s. The sensor also exhibits good reproducibility and stability. The sensor is selective for glucose even in the presence of 10-fold higher concentrations of ascorbic acid, lactic acid, dopamine, uric acid, and acetaminophen. Graphical abstract Schematic representation of the fabrication sequence for a nonenzymatic electrochemical glucose sensor using Nafion and platinum black coated microneedle electrode array. The sensor is based on measuring the faradaic current at +0.12 V vs. Ag/AgCl by the direct electrochemical oxidation of glucose to gluconic acid on the surface of a Pt black sensing layer.
作者报告了一种基于微针的安培非酶葡萄糖传感器，用于无痛和连续监测葡萄糖。它由 3×5 个锋利的不锈钢微针组成，这些微针是从不锈钢衬底微加工而成的。微针的高度和宽度分别为 600 和 100μm。金涂覆的微针尖端依次涂覆了 Nafion 和铂黑，用于非酶（直接）葡萄糖传感。修饰后的微针电极具有以下优点：(a) 工作电位低（+0.12V 对 Ag/AgCl）；(b) 在生理相关范围内呈线性响应（1-40mM）；(c) 灵敏度高达 175μA mM cm；(d) 检测限低至 23μM；(e) 响应时间为 2s。该传感器还具有良好的重现性和稳定性。即使在存在 10 倍以上浓度的抗坏血酸、乳酸、多巴胺、尿酸和对乙酰氨基酚的情况下，传感器对葡萄糖也具有选择性。
