Chinnadayyala Somasekhar R, Cho Sungbo
Department of Electronic Engineering, Gachon University, 1342 Seongnamdaero, Seongnam-si, Gyeonggi-do 13120, Korea.
Department of Health Science and Technology, GAIHST, Gachon University, Incheon 21999, Korea.
Nanomaterials (Basel). 2020 Dec 25;11(1):37. doi: 10.3390/nano11010037.
Individuals with diabetes can benefit considerably from continuous blood glucose monitoring. To address this challenge, a proof-of-concept was performed for continuous glucose monitoring (CGM) based on an enzymeless porous nanomaterial (pNM)-modified microneedle electrode array (MNEA). The pNM sensing layer was electrochemically deposited on MNs by applying a fixed negative current of -2.5 mA cm for 400 s. The pNM-modified MNEA was packed using a biocompatible Nafion ionomer. The fabricated MNEAs were 600 × 100 × 150 µm in height, width, and thickness, respectively. The surfaces of the modified MNs were characterized by scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). The fabricated MNEAs showed a wide dynamic range (1-30 mM) in phosphate-buffered saline (PBS) and in artificial interstitial fluid (ISF), with good sensitivities (PBS: 1.792 ± 0.25 µA mM cm, ISF: 0.957 ± 0.14 µA mM cm) and low detection limits (PBS: 7.2 µM, ISF: 22 µM). The sensor also showed high stability (loss of 3.5% at the end of 16 days), selectivity, and reproducibility (Relative standard deviations (RSD) of 1.64% and 0.70% for intra- and inter-assay, respectively) and a good response time (2 s) with great glucose recovery rates in ISF (98.7-102%).
糖尿病患者可从连续血糖监测中大幅获益。为应对这一挑战，基于无酶多孔纳米材料（pNM）修饰的微针电极阵列（MNEA）进行了连续血糖监测（CGM）的概念验证。通过施加-2.5 mA cm的固定负电流400 s，将pNM传感层电化学沉积在微针上。使用生物相容性的Nafion离聚物封装pNM修饰的MNEA。所制备的MNEA的高度、宽度和厚度分别为600×100×150 µm。通过扫描电子显微镜（SEM）、能量色散X射线分析（EDX）、X射线衍射（XRD）和X射线光电子能谱（XPS）对修饰微针的表面进行表征。所制备的MNEA在磷酸盐缓冲盐水（PBS）和人工间质液（ISF）中显示出较宽的动态范围（1-30 mM），具有良好的灵敏度（PBS：1.792±0.25 µA mM cm，ISF：0.957±0.14 µA mM cm）和低检测限（PBS：7.2 µM，ISF：22 µM）。该传感器还表现出高稳定性（16天结束时损失3.5%）、选择性和重现性（批内和批间相对标准偏差（RSD）分别为1.64%和0.70%）以及良好的响应时间（2 s），在ISF中的葡萄糖回收率很高（98.7-102%）。
