Zheng Mengjia, Zhang Yuyue, Hu Tianli, Xu Chenjie
Department of Biomedical Engineering City University of Hong Kong Hong Kong SAR People's Republic of China.
Bioeng Transl Med. 2022 Oct 10;8(5):e10413. doi: 10.1002/btm2.10413. eCollection 2023 Sep.
Microneedle (MN)-based diagnostic devices can efficiently access skin interstitial fluid (ISF) for accurate and minimally invasive detection of health-related biomarkers. This work reports a biomarker (i.e., glucose or alcohol) monitoring MN device that is composed of swellable MNs and electrochemical test strip. This device is constructed by adhering MN patch on the electrochemical strips using the chitosan as the connecting layer. The MNs penetrate the skin for extraction of ISF that flows to the backing layer of MNs and is analyzed by the test strip. In the in vitro skin models, this device accurately detects the glucose from 0 mM to 12 mM and alcohol from 0 mM to 20 mM. In vivo experiment shows this MN device is capable of minimally invasive sampling of ISF and analysis of glucose levels to determine the glycemic status of mice.
基于微针（MN）的诊断设备能够有效获取皮肤间质液（ISF），用于准确且微创地检测与健康相关的生物标志物。这项工作报道了一种生物标志物（即葡萄糖或酒精）监测微针设备，该设备由可膨胀微针和电化学测试条组成。此设备通过使用壳聚糖作为连接层，将微针贴片粘贴在电化学条上构建而成。微针穿透皮肤以提取ISF，ISF流到微针的背衬层并由测试条进行分析。在体外皮肤模型中，该设备能准确检测出浓度从0毫摩尔/升到12毫摩尔/升的葡萄糖以及浓度从0毫摩尔/升到20毫摩尔/升的酒精。体内实验表明，这种微针设备能够对ISF进行微创采样并分析葡萄糖水平，以确定小鼠的血糖状态。
