血浆外泌体在癌症患者诊断、预后及治疗中的新兴作用。
The emerging role of plasma exosomes in diagnosis, prognosis and therapies of patients with cancer.
作者信息
Whiteside Theresa L
机构信息
University of Pittsburgh Cancer Institute, Hillman Cancer Center, Pittsburgh, USA.
出版信息
Contemp Oncol (Pozn). 2018 Mar;22(1A):38-40. doi: 10.5114/wo.2018.73882. Epub 2018 Mar 5.
Abstract
Exosomes, small (30-150nm) extracellular vesicles of endocytic origin, are present in all body fluids of cancer patients. Tumor-derived exosomes, TEX, emerge as potentially promising non-invasive biomarkers of tumor progression and of immune cell dysfunction in cancer. Exosomes isolated from plasma by size exclusion chromatography can be fractionated into TEX and non-TEX by immune capture on beads. Profiling of molecular and genetic contents of TEX shows that levels if immunosuppressive proteins, such as PD-L1, carried by TEX associate with disease progression. The data suggest that TEX have a to serve as tumor surrogates, while immune cell-derived exosomes might serve as biomarkers of immune dysfunction in cancer.
摘要
外泌体是源自内吞作用的小（30 - 150纳米）细胞外囊泡，存在于癌症患者的所有体液中。肿瘤来源的外泌体（TEX）成为肿瘤进展和癌症中免疫细胞功能障碍潜在的有前景的非侵入性生物标志物。通过尺寸排阻色谱法从血浆中分离的外泌体可以通过磁珠免疫捕获分为TEX和非TEX。TEX分子和遗传内容的分析表明，TEX携带的免疫抑制蛋白如PD-L1的水平与疾病进展相关。数据表明TEX可作为肿瘤替代物，而免疫细胞来源的外泌体可能作为癌症中免疫功能障碍的生物标志物。
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