Institute of Chemical Biology and Nanomedicine, College of Chemistry and Chemical Engineering, Hunan University, 410082 Changsha, China.
Department of Chemistry and Chemical Biology, Northeastern University, Boston, MA 02115.
Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):4340-4344. doi: 10.1073/pnas.1801836115. Epub 2018 Apr 9.
Herein, we report a class of molecular spherical nucleic acid (SNA) nanostructures. These nano-sized single molecules are synthesized from T polyoctahedral silsesquioxane and buckminsterfullerene C scaffolds, modified with 8 and 12 pendant DNA strands, respectively. These conjugates have different DNA surface densities and thus exhibit different levels of nuclease resistance, cellular uptake, and gene regulation capabilities; the properties displayed by the C SNA conjugate are closer to those of conventional and prototypical gold nanoparticle SNAs. Importantly, the C SNA can serve as a single entity (no transfection agent required) antisense agent to efficiently regulate gene expression. The realization of molecularly pure forms of SNAs will open the door for studying the interactions of such structures with ligands and living cells with a much greater degree of control than the conventional polydisperse forms of SNAs.
在此，我们报告了一类分子球形核酸（SNA）纳米结构。这些纳米级的单分子是由 T 聚倍半硅氧烷和富勒烯 C 支架合成的，分别用 8 和 12 个悬垂 DNA 链修饰。这些缀合物具有不同的 DNA 表面密度，因此表现出不同程度的核酸酶抗性、细胞摄取和基因调控能力；C SNA 缀合物表现出的性质更接近传统和典型的金纳米颗粒 SNA。重要的是，C SNA 可以作为一种单一实体（无需转染剂）反义剂，有效地调节基因表达。分子纯形式的 SNA 的实现将为研究这些结构与配体和活细胞的相互作用打开大门，与传统的多分散形式的 SNA 相比，具有更大程度的控制。
