Department of Chemistry "Giacomo Ciamician", University of Bologna-Alma Mater Studiorum, Microchemistry and Microscopy Art Diagnostic Laboratory (M2ADL), Via Guaccimanni 42, 48121, Ravenna, Italy.
Department of Chemistry "Giacomo Ciamician", University of Bologna-Alma Mater Studiorum, Via Selmi 2, 40126, Bologna, Italy.
Angew Chem Int Ed Engl. 2018 Jun 18;57(25):7385-7389. doi: 10.1002/anie.201713298. Epub 2018 Apr 24.
The point-of-care testing concept has been exploited to design and develop portable and cheap bioanalytical systems that can be used on-site by conservators. These systems employ lateral flow immunoassays to simultaneously detect two proteins (ovalbumin and collagen) in artworks. For an in-depth study on the application of these portable biosensors, both chemiluminescent and colorimetric detections were developed and compared in terms of sensitivity and feasibility. The chemiluminescent system displayed the best analytical performance (that is, two orders of magnitude lower limits of detection than the colorimetric system). To simplify its use, a disposable cartridge was designed ad hoc for this specific application. These results highlight the enormous potential of these inexpensive, easy-to-use, and minimally invasive diagnostic tools for conservators in the cultural heritage field.
即时检测的概念已经被开发利用，用于设计和开发便携式、廉价的生物分析系统，这些系统可供保护人员现场使用。这些系统采用侧向流动免疫分析技术，同时检测艺术品中的两种蛋白质（卵白蛋白和胶原蛋白）。为了深入研究这些便携式生物传感器的应用，开发了化学发光和比色两种检测方法，并从灵敏度和可行性方面进行了比较。化学发光系统显示出最佳的分析性能（即检测限比比色系统低两个数量级）。为了简化其使用，专门为此特定应用设计了一次性试剂盒。这些结果突出了这些廉价、易于使用、微创诊断工具在文化遗产领域保护人员中的巨大潜力。
