Lee Katie Joanna, Clegg Peter David, Comerford Eithne Josephine, Canty-Laird Elizabeth Gail
Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease, University of Liverpool, William Henry Duncan Building, 6 West Derby Street, Liverpool, L7 8TX, UK.
School of Veterinary Science, Leahurst Campus, University of Liverpoo, Chester High Road, Neston, CH64 7TE, UK.
BMC Musculoskelet Disord. 2018 Apr 12;19(1):116. doi: 10.1186/s12891-018-2038-2.
Tendon is a commonly injured soft musculoskeletal tissue, however, poor healing potential and ineffective treatment strategies result in persistent injuries and tissue that is unable to perform its normal physiological function. The identification of a stem cell population within tendon tissue holds therapeutic potential for treatment of tendon injuries. This study aimed, for the first time, to characterise and compare tenocyte and tendon-derived stem cell (TDSC) populations in murine tendon. Tenocytes and TDSCs were isolated from murine tail tendon. The cells were characterised for morphology, clonogenicity, proliferation, stem cell and tenogenic marker expression and multipotency. TDSCs demonstrated a rounded morphology, compared with a more fibroblastic morphology for tenocytes. Tenocytes had greater clonogenic potential and a smaller population doubling time compared with TDSCs. Stem cell and early tenogenic markers were more highly expressed in TDSCs, whereas late tenogenic markers were more highly expressed in tenocytes. Multipotency was increased in TDSCs with the presence of adipogenic differentiation which was absent in tenocytes. The differences in morphology, clonogenicity, stem cell marker expression and multipotency observed between tenocytes and TDSCs indicate that at least two cell populations are present in murine tail tendon. Determination of the most effective cell population for tendon repair is required in future studies, which in turn may aid in tendon repair strategies.
肌腱是一种常见的易损伤的肌肉骨骼软组织，然而，其愈合潜力差和治疗策略无效导致损伤持续存在，且组织无法执行其正常生理功能。在肌腱组织中鉴定出干细胞群体为治疗肌腱损伤具有治疗潜力。本研究首次旨在表征和比较小鼠肌腱中的腱细胞和肌腱来源干细胞（TDSC）群体。从鼠尾肌腱中分离出腱细胞和TDSC。对这些细胞进行形态学、克隆形成能力、增殖、干细胞和肌腱生成标记物表达以及多能性的表征。与腱细胞更呈成纤维细胞形态相比，TDSC表现出圆形形态。与TDSC相比，腱细胞具有更大的克隆形成潜力和更短的群体倍增时间。干细胞和早期肌腱生成标记物在TDSC中表达更高，而晚期肌腱生成标记物在腱细胞中表达更高。在存在脂肪生成分化的情况下，TDSC的多能性增加，而腱细胞中不存在这种情况。腱细胞和TDSC之间在形态学、克隆形成能力、干细胞标记物表达和多能性方面的差异表明，鼠尾肌腱中至少存在两个细胞群体。未来的研究需要确定用于肌腱修复的最有效细胞群体，这反过来可能有助于肌腱修复策略。
