Key Laboratory of Luminescent and Real-Time Analytical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering , Southwest University , Chongqing 400715 , PR China.
Anal Chem. 2018 May 1;90(9):5945-5950. doi: 10.1021/acs.analchem.8b00894. Epub 2018 Apr 18.
In situ monitoring of hydrogen peroxide (HO) secreted from live cells plays a critical role in elucidating many cellular signaling pathways, and it is a significant challenge to selectively detect these low levels of endogenous HO. To address this challenge, we report the establishment of a trimetallic hybrid nanoflower-decorated MoS nanosheet-modified sensor for in situ monitoring of HO secreted from live MCF-7 cancer cells. The Au-Pd-Pt nanoflower-dispersed MoS nanosheets are synthesized by a simple wet-chemistry method, and the resulting nanosheet composites exhibit significantly enhanced catalytic activity toward electrochemical reduction of HO, due to the synergistic effect of the highly dispersed trimetallic hybrid nanoflowers and the MoS nanosheets, thereby resulting in ultrasensitive detection of HO with a subnanomolar level detection limit in vitro. Also the immobilization of the laminin glycoproteins on the surface of the nanocomposites increases its biocompatibility for cell adhesion and growth, which enables in situ electrochemical monitoring of HO directly secreted from live cells for potential application of such sensor in cellular biology, clinical diagnosis, and pathophysiology.
原位监测活细胞分泌的过氧化氢（HO）在阐明许多细胞信号通路方面起着至关重要的作用，而选择性检测这些低水平的内源性 HO 是一项重大挑战。为了解决这一挑战，我们报告了一种三金属混合纳米花修饰的 MoS 纳米片修饰传感器的建立，用于原位监测活 MCF-7 癌细胞分泌的 HO。Au-Pd-Pt 纳米花分散的 MoS 纳米片是通过简单的湿化学方法合成的，由于高度分散的三金属混合纳米花和 MoS 纳米片的协同作用，所得纳米片复合材料对 HO 的电化学还原表现出显著增强的催化活性，从而实现了 HO 的超灵敏检测，在体外具有亚纳摩尔级别的检测限。此外，层粘连蛋白糖蛋白固定在纳米复合材料的表面上增加了其对细胞黏附和生长的生物相容性，这使得可以直接从活细胞中原位电化学监测 HO，从而为这种传感器在细胞生物学、临床诊断和病理生理学中的潜在应用提供了可能。
