Turcotte Raphaël, Liang Yajie, Ji Na
Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA.
Biomed Opt Express. 2017 Jul 31;8(8):3891-3902. doi: 10.1364/BOE.8.003891. eCollection 2017 Aug 1.
Adjusting the objective correction collar is a widely used approach to correct spherical aberrations (SA) in optical microscopy. In this work, we characterized and compared its performance with adaptive optics in the context of brain imaging with two-photon fluorescence microscopy. We found that the presence of sample tilt had a deleterious effect on the performance of SA-only correction. At large tilt angles, adjusting the correction collar even worsened image quality. In contrast, adaptive optical correction always recovered optimal imaging performance regardless of sample tilt. The extent of improvement with adaptive optics was dependent on object size, with smaller objects having larger relative gains in signal intensity and image sharpness. These observations translate into a superior performance of adaptive optics for structural and functional brain imaging applications , as we confirmed experimentally.
调整物镜校正环是光学显微镜中广泛用于校正球差（SA）的一种方法。在这项工作中，我们在双光子荧光显微镜脑成像的背景下，对其性能进行了表征，并与自适应光学进行了比较。我们发现样品倾斜的存在对仅SA校正的性能有有害影响。在大倾斜角度下，调整校正环甚至会使图像质量变差。相比之下，无论样品倾斜如何，自适应光学校正总能恢复最佳成像性能。自适应光学的改进程度取决于物体大小，较小的物体在信号强度和图像清晰度方面具有更大的相对增益。正如我们通过实验所证实的，这些观察结果表明自适应光学在脑结构和功能成像应用中具有卓越的性能。
