School of Health Studies, University of Memphis, Memphis, TN, United States; Center for Nutraceutical and Dietary Supplement Research, University of Memphis, Memphis, TN, United States.
School of Health Studies, University of Memphis, Memphis, TN, United States.
Exp Gerontol. 2018 Jul 15;108:112-117. doi: 10.1016/j.exger.2018.04.008. Epub 2018 Apr 13.
Aging is a critical healthcare concern, with age-related inflammation disposing individuals to a variety of diseases. Monocytes are affected by the aging process, with increased inflammation and impaired cellular functions such as phagocytosis. Mechanisms by which aging alters monocyte function are unknown, but recent research suggests that the balance of metabolic processes determine immune cell phenotype and function. Given the known association between aging and mitochondrial dysfunction in other tissues, we hypothesized that aging would impair mitochondrial function in monocytes. To test this, we isolated classical monocytes from young and older adults and tested mitochondrial function by a Seahorse assay. Aging reduced mitochondrial respiratory capacity and spare capacity in monocytes. Mitochondrial dysfunction is a potential mechanism by which aging alters monocyte phenotype and may impair inflammatory functions, especially in low-glucose environments where oxidative metabolism is necessary to meet energy demands.
衰老是一个关键的医疗保健问题，与年龄相关的炎症使个体易患多种疾病。单核细胞受衰老过程的影响，炎症增加，吞噬等细胞功能受损。衰老改变单核细胞功能的机制尚不清楚，但最近的研究表明，代谢过程的平衡决定了免疫细胞的表型和功能。鉴于其他组织中衰老与线粒体功能障碍之间的已知关联，我们假设衰老将损害单核细胞中的线粒体功能。为了验证这一点，我们从小龄和大龄成年人中分离出经典单核细胞，并通过 Seahorse 测定法测试线粒体功能。衰老降低了单核细胞中线粒体的呼吸能力和备用能力。线粒体功能障碍是衰老改变单核细胞表型的潜在机制，并可能损害炎症功能，尤其是在需要氧化代谢来满足能量需求的低糖环境中。
