叶片提取物对虫卵的杀卵活性比较
Comparative ovicidal activity of leaf extracts on eggs.
作者信息
Hegazi Ahmed G, Megeed Kadria N Abdel, Hassan Soad E, Abdelaziz M M, Toaleb Nagwa I, Shanawany Eman E El, Aboelsoued Dina
机构信息
Department of Zoonotic Diseases, National Research Centre, Dokki, Giza, Egypt.
Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Giza, Egypt.
出版信息
Vet World. 2018 Feb;11(2):215-220. doi: 10.14202/vetworld.2018.215-220. Epub 2018 Feb 19.
BACKGROUND
Fasciolosis is an important zoonotic disease affecting the productive performance of farm animals in Egypt.
AIM
The aim of the present study was comparing the ovicidal effect of different extracts as an alcoholic (Methanolic and Ethanolic) and aqueous leaf extracts on non-embryonated and developed eggs.
MATERIALS AND METHODS
Tested concentrations of extracts ranged from 12.5 to 800 mg/ml. Nitroxynil was used as reference drug with a dose of 100 mg/ml.
RESULTS
alcoholic and aqueous extracts showed a concentration-dependent ovicidal effect on non-embryonated and developed eggs. Based on LC values, water extract showed the highest ovicidal activity since it registered the lowest values of 2.6 mg/ml on non-embryonated eggs. Non-embryonated eggs were more susceptible to aqueous extract than developed eggs. On the other hand, the developed eggs were more susceptible to ethanolic extract than non-embryonated eggs even the lowest LC (12.38 mg/ml).
CONCLUSION
leaf extracts especially aqueous extract could be a promising step in the field of controlling fascioliasis. Further, studies are needed to enlighten the therapeutic potential of extracts in treating infection.
背景
肝片吸虫病是一种重要的人畜共患病，影响埃及农场动物的生产性能。
目的
本研究的目的是比较不同提取物（醇提取物（甲醇提取物和乙醇提取物）和水提物）对未受精卵和已发育卵的杀卵效果。
材料与方法
提取物的测试浓度范围为12.5至800毫克/毫升。硝羟碘苄腈用作参考药物，剂量为100毫克/毫升。
结果
醇提取物和水提取物对未受精卵和已发育卵均表现出浓度依赖性杀卵效果。基于半数致死浓度（LC）值，水提取物显示出最高的杀卵活性，因为它在未受精卵上的最低值为2.6毫克/毫升。未受精卵比已发育卵对水提取物更敏感。另一方面，已发育卵比未受精卵对乙醇提取物更敏感，即使最低半数致死浓度（12.38毫克/毫升）也是如此。
结论
叶提取物尤其是水提取物可能是控制肝片吸虫病领域的一个有前景的措施。此外，需要进一步研究以阐明提取物在治疗感染方面的治疗潜力。
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