Sharma H S, Dey P K
Department of Physiology, Banaras Hindu University, Varanasi, India.
Neurosci Res. 1988 Feb;5(3):224-39. doi: 10.1016/0168-0102(88)90051-x.
A continuous 8 h of immobilization stress in conscious young rats increased the blood-brain barrier (BBB) permeability to 131I-sodium in 12 out of 14 brain regions studied. A flattening of electroencephalographic (EEG) activity was noted during this time period. The mean cerebral blood flow (CBF) was reduced by 17% (during this time period) but the regional flow reduction was not related to the regional increase in BBB permeability. On the other hand, a correlation was observed between increased plasma and brain 5-HT levels and increased BBB permeability. p-Chloro-phenylalanine (p-CPA) pretreatment has prevented the occurrence of increased BBB permeability, and the flattening of EEG activity as well as 5-HT levels in plasma and brain. These results suggest that the long-term immobilization stress induces causally related sequential events in rats: enhancement of circulating 5-HT, impairment of BBB, free access of 5-HT into the brain, and eventually flattening of EEG.
对清醒幼鼠连续施加8小时的制动应激，在所研究的14个脑区中的12个脑区，血脑屏障（BBB）对131I - 钠的通透性增加。在此期间观察到脑电图（EEG）活动变平。平均脑血流量（CBF）降低了17%（在此期间），但局部血流量的减少与血脑屏障通透性的局部增加无关。另一方面，观察到血浆和脑内5 - 羟色胺（5 - HT）水平升高与血脑屏障通透性增加之间存在相关性。对氯苯丙氨酸（p - CPA）预处理可防止血脑屏障通透性增加、脑电图活动变平以及血浆和脑内5 - HT水平升高的发生。这些结果表明，长期制动应激在大鼠中诱发了因果相关的连续事件：循环5 - HT增强、血脑屏障受损、5 - HT自由进入脑内，最终脑电图变平。
