Zhang Bin, Chu Nan, Qiu Xue-Min, Tang Wei, Gober Hans-Jürgen, Li Da-Jin, Wang Ling
Institute of Obstetrics and Gynecology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai, 200011, China.
The Academy of Integrative Medicine of Fudan University, Shanghai, 200011, China.
Chin J Integr Med. 2018 Oct;24(10):768-776. doi: 10.1007/s11655-018-2835-3. Epub 2018 Apr 17.
To evaluate the effect of Heyan Kuntai Capsule (, HYKT) on the ovarian function of aged mice and expressions of cohesion complexes in oocytes.
Twenty-five 9-month-old female C57BL/6J mice were randomly divided into 5 groups by block randomization method (n=5 per group), including the control group (saline), 17β-estradiol group [E, 100 μg/(kg•d)], and low-, medium-, and highdose of HYKT groups [0.3, 0.9, 2.7 g/(kg•d), respectively]. All mice were treated by intragastric administration for 4 weeks. Hematoxylin and eosin staining and anti-VASA staining were used to detect the amounts of follicles. The apoptosis of follicles was measured by anti-gamma HA histone family member X (γHAX) staining and TdT-mediated dUTP Nick-End Labeling (TUNEL) assay. The density of cohesin subunits, REC8 meiotic recombination protein (REC8), structural maintenance of chromosome (SMC) 1β and SMC3 in oocytes were evaluated by immunofluorescent staining.
After administration of E and high-dose of HYKT, the total number of follicles as well as the number of primordial and primary follicles were significantly increased (P<0.05). Anti-γHAX staining and TUNEL assay demonstrated that high-dose of HYKT and E partly suppressed the apoptosis of follicles (P<0.05). Furthermore, it showed an increased trend in the levels of REC8 and SMC1β, after administration with E and HYKT, and no obvious change in the level of SMC3.
HYKT could enhance the number of follicles, suppress apoptosis of oocytes and have a trend to elevate the meiotic-specific cohesin subunits (REC8 and SMC1β) in oocytes of aged mice, indicating a beneficial effect on the ovarian function in terms of the quantity and quality of follicles.
评估和颜坤泰胶囊（HYKT）对老年小鼠卵巢功能及卵母细胞中黏连复合体表达的影响。
采用区组随机化法将25只9月龄雌性C57BL/6J小鼠随机分为5组（每组n = 5），包括对照组（生理盐水）、17β - 雌二醇组[E，100 μg/(kg•d)]以及低、中、高剂量HYKT组[分别为0.3、0.9、2.7 g/(kg•d)]。所有小鼠经灌胃给药4周。采用苏木精 - 伊红染色和抗VASA染色检测卵泡数量。通过抗γH2AX组蛋白家族成员X（γHAX）染色和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）法检测卵泡凋亡情况。采用免疫荧光染色评估卵母细胞中黏连蛋白亚基、减数分裂重组蛋白REC8、染色体结构维持蛋白（SMC）1β和SMC3的密度。
给予E和高剂量HYKT后，卵泡总数以及原始卵泡和初级卵泡数量显著增加（P<0.05）。抗γHAX染色和TUNEL检测表明，高剂量HYKT和E部分抑制了卵泡凋亡（P<0.05）。此外，给予E和HYKT后，REC8和SMC1β水平呈上升趋势，而SMC3水平无明显变化。
HYKT可增加老年小鼠卵泡数量，抑制卵母细胞凋亡，并使卵母细胞中减数分裂特异性黏连蛋白亚基（REC8和SMC1β）有升高趋势，提示其在卵泡数量和质量方面对卵巢功能有有益影响。
