Hwang Moon-Jin, Kim Bong-Jun, Song Yo-Han, Song Ho-Jun, Park Yeong-Joon
J Nanosci Nanotechnol. 2017 Apr;17(4):2828-832. doi: 10.1166/jnn.2017.13330.
The surface characteristics and osteoconductivity were evaluated for the micro/nanoporous surfaces of titanium (Ti) alloys prepared by micro-arc oxidation (MAO) and hydrothermal treatment (HT) of binary Ti-5 wt% A alloys (A = Au, Mn, Nb, and Pd). Surface properties were analyzed using X-ray diffractometry, scanning electron microscopy, and energy dispersive X-ray spectroscopy. The osteoconductivity was evaluated by measuring the total protein, ALPase activity, and osteocalcin production. The surface morphologies of MAO/HT specimens mainly affected on their osteoconductivity. Total proteins on Ti alloys (MAO/HT) were slightly lower than that on commercially pure Ti (MAO/HT) after incubation of MG-63 osteoblast-like cells for 14 days. However, better ALPase activity and osteocalcin production were observed on MAO/HT-treated Ti–5Mn, Ti–5Nb, and Ti–5Pd than that on cp-Ti (MAO/HT) after 14 days. Especially, Ti–5Mn (MAO/HT) showed a significant increase of ALPase activity due to its well grown micro/nano structure. Meanwhile, very small nanorods on Ti–5Au (MAO/HT) affected negatively to ALPase activity and osteocalcin production.
对通过微弧氧化（MAO）和水热法（HT）制备的二元Ti-5 wt% A合金（A = Au、Mn、Nb和Pd）的钛（Ti）合金微/纳米多孔表面的表面特性和骨传导性进行了评估。使用X射线衍射、扫描电子显微镜和能量色散X射线光谱分析表面性能。通过测量总蛋白、碱性磷酸酶（ALPase）活性和骨钙素生成量来评估骨传导性。MAO/HT试样的表面形态主要影响其骨传导性。在MG-63成骨样细胞培养14天后，Ti合金（MAO/HT）上的总蛋白略低于商业纯钛（MAO/HT）上的总蛋白。然而，在14天后，观察到MAO/HT处理的Ti-5Mn、Ti-5Nb和Ti-5Pd上的ALPase活性和骨钙素生成量比cp-Ti（MAO/HT）上的更好。特别是，Ti-5Mn（MAO/HT）由于其良好生长的微/纳米结构而显示出ALPase活性显著增加。同时，Ti-5Au（MAO/HT）上非常小的纳米棒对ALPase活性和骨钙素生成有负面影响。
