（林奈）对大鼠野百合碱诱导的肺动脉高压的有益作用。
Beneficial Effect of (Linn) against Monocrotaline-Induced Pulmonary Hypertension in Rats.
作者信息
Meghwani Himanshu, Prabhakar Pankaj, Mohammed Soheb A, Dua Pamila, Seth Sandeep, Hote Milind P, Banerjee Sanjay K, Arava Sudheer, Ray Ruma, Maulik Subir Kumar
机构信息
Department of Pharmacology, All India Institute of Medical Sciences (AIIMS), New Delhi 110029, India.
Drug Discovery Research Center (DDRC), Translational Health Science and Technology Institute (THSTI), Faridabad 121001, India.
出版信息
Medicines (Basel). 2018 Apr 17;5(2):34. doi: 10.3390/medicines5020034.
BACKGROUND
The study was designed to explore any beneficial effect of Ocimum sanctum (Linn) (OS) in experimental pulmonary hypertension (PH) in rats. OS is commonly known as “holy basil” and “Tulsi” and is used in the Indian System of Medicine as antidiabetic, antioxidant, hepatoprotective, adaptogenic, and cardioprotective.
METHODS
Monocrotaline (MCT) administration caused development of PH in rats after 28 days and rats were observed for 42 days. Treatments (sildenafil; 175 µg/kg, OS; 200 mg/kg) were started from day 29 after the development of PH and continued for 14 days. Parameters to assess the disease development and effectiveness of interventions were echocardiography, right and left ventricular systolic pressures, and right ventricular end diastolic pressure, percentage medial wall thickness (%MWT) of pulmonary artery, oxidative stress markers in lung tissue, NADPH oxidase (Nox-1) protein expression in lung, and mRNA expression of Bcl2 and Bax in right ventricular tissue.
RESULTS
OS (200 mg/kg) treatment ameliorated increased lung weight to body weight ratio, right ventricular hypertrophy, increased RVSP, and RVoTD/AoD ratio. Moreover, OS treatment decreases Nox-1 expression and increases expression of Bcl2/Bax ratio caused by MCT.
CONCLUSION
The present study demonstrates that OS has therapeutic ability against MCT-induced PH in rat which are attributed to its antioxidant effect. The effect of OS was comparable with sildenafil.
背景
本研究旨在探讨神圣罗勒（Ocimum sanctum (Linn)，OS）对大鼠实验性肺动脉高压（PH）是否具有有益作用。OS通常被称为“圣罗勒”和“图尔西”，在印度医学体系中用作抗糖尿病、抗氧化、保肝、适应原和心脏保护剂。
方法
给予大鼠野百合碱（MCT）28天后诱发PH，并对大鼠观察42天。在PH发生后的第29天开始治疗（西地那非；175μg/kg，OS；200mg/kg），持续14天。评估疾病发展和干预效果的参数包括超声心动图、左右心室收缩压、右心室舒张末期压力、肺动脉中层壁厚度百分比（%MWT）、肺组织中的氧化应激标志物、肺组织中NADPH氧化酶（Nox-1）蛋白表达以及右心室组织中Bcl2和Bax的mRNA表达。
结果
OS（200mg/kg）治疗改善了肺重量与体重比增加、右心室肥大、右心室收缩压升高以及右心室舒张末期内径与主动脉内径比值升高的情况。此外，OS治疗降低了Nox-1表达，并增加了由MCT引起的Bcl2/Bax比值的表达。
结论
本研究表明，OS对MCT诱导的大鼠PH具有治疗能力，这归因于其抗氧化作用。OS的效果与西地那非相当。
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