Department of Agriculture Chemistry, Chonbuk National University, Jeollabuk-do 561-756, South Korea.
Department of Agriculture Chemistry, Chonbuk National University, Jeollabuk-do 561-756, South Korea.
Sci Total Environ. 2018 Sep 1;635:364-374. doi: 10.1016/j.scitotenv.2018.04.101. Epub 2018 Apr 24.
Plant-veterinary antibiotic interaction has been widely studied, however, to the best of our knowledge acclimatization studies with regard to changes in plant root metabolites has not been reported so far. The purpose of this study was to examine the changes in the metabolome of pea roots under antibiotic stress and their role in acclimatization. Pisum sativum L. was grown in soil contaminated with three commonly used veterinary antibiotics - kanamycin (KA), sulfamethazine (SA), and tetracycline (TC). In response to antibiotic stress, plants accumulated different types of low molecular weight compounds that provided protection from stress by contributing to ROS detoxification, protection of membrane integrity, efficient signaling, cell wall function, and cellular osmotic adjustment (glucose, galactose, myo-inositol, stigmasterol, octadecadienoic acid, l-proline). The concentration of amino acid, sugar, and triglyceride metabolites in KA and TC samples showed a dose-dependent biphasic (hormesis) fluctuation. This was mirrored in the metabolite abundance as well as the physiological attributes (mycorrhizal colonization, GST function, nutrient assimilation), which helped in the acclimatization without the loss of normal plant function. SA, on the other hand, had progressive toxic effects with increasing concentration. PCA revealed the differences to be due to SA treatments and in sterol and terpenoid metabolites.
植物-兽医抗生素相互作用已得到广泛研究，但据我们所知，目前尚未有关于植物根系代谢物变化适应驯化的研究。本研究旨在探讨抗生素胁迫下豌豆根系代谢组的变化及其在适应驯化中的作用。将豌豆（Pisum sativum L.）种植在受三种常用兽医抗生素（卡那霉素（KA）、磺胺甲噁唑（SA）和四环素（TC））污染的土壤中。在抗生素胁迫下，植物积累了不同类型的低分子量化合物，这些化合物通过有助于 ROS 解毒、保护膜完整性、高效信号转导、细胞壁功能和细胞渗透调节（葡萄糖、半乳糖、肌醇、豆甾醇、十八碳二烯酸、l-脯氨酸）来提供保护，从而适应胁迫。KA 和 TC 样品中氨基酸、糖和三酰基甘油代谢物的浓度表现出剂量依赖性双相（适应原）波动。这反映在代谢物丰度以及生理特性（菌根定殖、GST 功能、养分吸收）上，这有助于适应而不丧失正常植物功能。另一方面，SA 随着浓度的增加表现出渐进性的毒性作用。PCA 表明差异是由于 SA 处理以及固醇和萜类代谢物引起的。
