Faculty of Food Sciences, Department of Biotechnology, Microbiology and Food Evaluation, Warsaw University of Life Sciences - SGGW, Nowoursynowska 159 C, 02-776, Warsaw, Poland.
Biol Trace Elem Res. 2019 Jan;187(1):316-327. doi: 10.1007/s12011-018-1342-x. Epub 2018 Apr 19.
This article discusses the effect of selenium in aqueous solutions on aspects of lipid and amino acid metabolism in the cell biomass of Saccharomyces cerevisiae MYA-2200 and Candida utilis ATCC 9950 yeasts. The yeast biomass was obtained by using waste products (potato wastewater and glycerol). Selenium, at a dose of 20 mg/L of aqueous solution, affected the differentiation of cellular morphology. Yeast enriched with selenium was characterized by a large functional diversity in terms of protein and amino acid content. The protein content in the biomass of S. cerevisiae enriched with selenium (42.6%) decreased slightly as compared to that in the control sample without additional selenium supplementation (48.4%). Moreover, yeasts of both strains enriched with selenium contained a large amount of glutamic acid, aspartic acid, lysine, and leucine. Analysis of fatty acid profiles in S. cerevisiae yeast supplemented with selenium showed an increase in the unsaturated fatty acid content (e.g., C18:1). The presence of margaric acid (C17:0) and hexadecanoic acid (C17:1) was found in the C. utilis biomass enriched with selenium, in contrast to that of S. cerevisiae. These results indicate that selenium may induce lipid peroxidation, which consequently affects the loss of integrity of the cytoplasmic membrane. Yeast enriched with selenium with optimal amino acid and lipid composition can be used to prepare a novel formula of dietary supplements, which can be applied directly to various diets for both humans and animals.
本文讨论了硒在水溶液中对酿酒酵母 MYA-2200 和产朊假丝酵母 ATCC 9950 细胞生物量中脂质和氨基酸代谢方面的影响。酵母生物量是使用废物（土豆废水和甘油）获得的。硒的剂量为 20mg/L 的水溶液，影响了细胞形态的分化。富含硒的酵母在蛋白质和氨基酸含量方面表现出很大的功能多样性。与未添加额外硒的对照样品（48.4%）相比，富含硒的 S. cerevisiae 生物质中的蛋白质含量（42.6%）略有下降。此外，两种菌株的富硒酵母都含有大量的谷氨酸、天冬氨酸、赖氨酸和亮氨酸。对添加硒的 S. cerevisiae 酵母的脂肪酸谱进行分析表明，不饱和脂肪酸的含量增加（例如，C18:1）。在富含硒的 C. utilis 生物质中发现了花生酸（C17:0）和十六烷酸（C17:1），而 S. cerevisiae 中则没有。这些结果表明，硒可能诱导脂质过氧化，从而影响细胞质膜完整性的丧失。富含硒、具有最佳氨基酸和脂质组成的酵母可用于制备新型膳食补充剂配方，可直接应用于人类和动物的各种饮食。
