Arous Fatma, Azabou Samia, Triantaphyllidou Irene-Eva, Aggelis George, Jaouani Atef, Nasri Moncef, Mechichi Tahar
Laboratory of Enzyme Engineering and Microbiology National School of Engineers of Sfax University of Sfax Sfax Tunisia.
Laboratory of Valorization Security and Food Analysis National School of Engineers of Sfax University of Sfax Sfax Tunisia.
Eng Life Sci. 2016 Mar 30;17(3):226-236. doi: 10.1002/elsc.201500156. eCollection 2017 Mar.
Newly isolated yeasts from different Tunisian microhabitats, such as soil, milk, olive brine, vinegar, and from olive mill wastewater-contaminated biotopes were extensively studied for their biochemical arsenal and morphological features, i.e. cell, ascospore, and lipid body morphology. All strains were classified into the Ascomycota phylum. However, they showed great functional diversity, including different morphological and biochemical features, lipid production ability, and fatty acid profiles. Accordingly, the strains were placed in three different groups: Group I, which includes species; Group II ( and related); and Group III ( strain CC1). Group I and II were characterized by a high percentage of oleic acid (41.6-65.3% of total lipids) while in Group III, linoleic acid was the major fatty acid (37.2%). Members of Group I and II were further grouped into subgroups according to their fatty acid composition. Among the newly isolated strains, BM1 was able to accumulate around 25% wt/wt lipid per dry cell mass and thus characterized as oleaginous. Some other strains, such as strain EL2, strain LV2, strain BM24, and strain CC1, which are able to produce extracellular lipases, may be of interest for specific environmental applications and/or for the production of novel lipases.
对从突尼斯不同微生境（如土壤、牛奶、橄榄盐水、醋）以及受橄榄榨油厂废水污染的生物群落中新分离出的酵母进行了广泛研究，分析了它们的生化特性和形态特征，即细胞、子囊孢子和脂质体形态。所有菌株均归类于子囊菌门。然而，它们表现出极大的功能多样性，包括不同的形态和生化特征、脂质生产能力以及脂肪酸谱。因此，这些菌株被分为三个不同的组：第一组，包括 种；第二组（ 及相关种）；以及第三组（ 菌株CC1）。第一组和第二组的特征是油酸含量较高（占总脂质的41.6 - 65.3%），而在第三组中，亚油酸是主要脂肪酸（37.2%）。第一组和第二组的成员根据其脂肪酸组成进一步分为亚组。在新分离的菌株中， BM1每干细胞质量能够积累约25%（重量/重量）的脂质，因此被表征为产油酵母。其他一些菌株，如 菌株EL2、 菌株LV2、 菌株BM24和 菌株CC1，能够产生细胞外脂肪酶，可能在特定环境应用和/或新型脂肪酶的生产方面具有应用价值。
