Unidad Académica de Sistemas Arrecifales, Puerto Morelos, Instituto de Ciencias del Mar y Limnología, Universidad Nacional Autónoma de México, Puerto Morelos, Quintana Roo State, Mexico.
J Venom Anim Toxins Incl Trop Dis. 2013 May 7;19(1):12. doi: 10.1186/1678-9199-19-12.
Lung cancer causes 1.4 million deaths worldwide while non-small-cell lung cancer (NSCLC) represents 80-85% of the cases. Cisplatin is a standard chemotherapy against this type of cancer; however, tumor cell resistance to this drug limits its efficacy. Sea anemones produce compounds with pharmacological activities that may be useful for augmenting cisplatin efficacy. This study aimed to evaluate the pharmacological activities of crude venom (CV) from the sea anemone Bunodeopsis globulifera and four derived fractions (F1, F2, F3 and F4) to test their increase efficiency cisplatin cytotoxicity in human lung adenocarcinoma cells.
Pre-exposure to CV, F1 and F2 fractions increases cisplatin cytotoxicity in human lung adenocarcinoma cells under specific conditions. Exposure to CV at 50 μgmL-1 induced a reduction of approximately 50% in cell viability, while a similar cytotoxic effect was observed when cell culture was exposed to F1 at 25 μgmL -1 or F2 at 50 μgmL-1. The cell culture exposure to F1 (10 μgmL-1) fraction combined with cisplatine (25 μM) provoked a decrease in MTT reduction until 65.57% while F2 (25 μgmL-1) fraction combined with cisplatin (10 μM) provoked a decrease in MTT reduction of 72.55%.
The F1 fraction had the greatest effect on the lung adenocarcinoma cell line compared with CV and F2. The combination of antineoplastic drugs and sea anemone toxins might allow a reduction of chemotherapeutic doses and thus mitigate side effects.
肺癌导致全球 140 万人死亡，而非小细胞肺癌（NSCLC）占病例的 80-85%。顺铂是治疗这种癌症的标准化疗药物；然而，肿瘤细胞对这种药物的耐药性限制了其疗效。海葵产生具有药理活性的化合物，可能有助于增强顺铂的疗效。本研究旨在评估海葵 Bunodeopsis globulifera 的粗毒液（CV）及其四个衍生部分（F1、F2、F3 和 F4）的药理活性，以测试它们对人肺腺癌细胞中顺铂细胞毒性的增效作用。
在特定条件下，CV、F1 和 F2 部分预先暴露于 CV 可增加人肺腺癌细胞中顺铂的细胞毒性。CV 在 50μgmL-1 的浓度下暴露可使细胞活力降低约 50%，而当细胞培养物暴露于 F1 在 25μgmL-1 或 F2 在 50μgmL-1 时观察到类似的细胞毒性作用。F1（10μgmL-1）部分与顺铂（25μM）联合暴露于细胞培养物可使 MTT 还原减少至 65.57%，而 F2（25μgmL-1）部分与顺铂（10μM）联合暴露于细胞培养物可使 MTT 还原减少 72.55%。
与 CV 和 F2 相比，F1 部分对肺腺癌细胞系的影响最大。抗肿瘤药物与海葵毒素的联合使用可能会降低化疗药物的剂量，从而减轻副作用。
