Bastani A, Asghary A, Heidari M H, Karimi-Busheri F
Eur J Gynaecol Oncol. 2017;38(3):418-424.
The aim of this work was to compare and analyze the diagnostic value of serum prostasin, cancer antigen 125 (CA125), lactate dehydrogenase (LDH), alpha-fetoprotein (AFP), and human chorionic gonadotropin (hCG+β) in epithelial ovarian cancer (EOC) and evaluate if their serum levels could be used as a potential diagnostic markers of EOC from benign tumors and healthy women.
Preoperative serum samples of 110 women (24 healthy controls, 66 ovarian benign tumors, and 20 EOC) were tested for prostasin, CA125, AFP, and hCG+β. The level of CA125, AFP, and hCG+β serum tumor markers were determined by electro-chemiluminescence immunoassay (ECLIA) and the serum level of prostasin was measured using enzyme-linked immunosorbent assay (ELISA) and LDH activity was measured by spectrophotometer and analyzed using SPSS version.
The Area Under the Curve (AUC) values of prostasin, CA125, LDH, AFP, and hCG+β for the discrimination of EOC from benign and healthy controls were, respectively, 0.89, 0.91, 0.77, 0.54, and 0.65, and significant increase in serum levels of prostasin, CA125, and LDH were observed for EOC compared with benign and control groups.
The present study showed that CA 125 and LDH levels of serum increased in high stages, while prostasin level was decreased in high stages. The present results indicate that prostasin, CA125, and LDH are differentially expressed in EOC than in benign and healthy control population, that may be an indicative of a better diagnostic value, with higher sen- sitivity and specificity. Here the authors used a multimarker approach, consisting of CA125, AFP, beta hCG, prostasin, and LDH that could provide a more accurate tool for a differential diagnosis of patients with EOC.
本研究旨在比较和分析血清前列腺素酶、癌抗原125（CA125）、乳酸脱氢酶（LDH）、甲胎蛋白（AFP）和人绒毛膜促性腺激素（hCG+β）在上皮性卵巢癌（EOC）中的诊断价值，并评估其血清水平是否可作为EOC与良性肿瘤及健康女性的潜在诊断标志物。
对110名女性（24名健康对照者、66名卵巢良性肿瘤患者和20名EOC患者）的术前血清样本进行前列腺素酶、CA125、AFP和hCG+β检测。采用电化学发光免疫分析法（ECLIA）测定CA125、AFP和hCG+β血清肿瘤标志物水平，采用酶联免疫吸附测定法（ELISA）测定前列腺素酶血清水平，用分光光度计测定LDH活性，并使用SPSS软件进行分析。
前列腺素酶、CA125、LDH、AFP和hCG+β用于区分EOC与良性及健康对照者的曲线下面积（AUC）值分别为0.89、0.91、0.77、0.54和0.65，与良性及对照组相比，EOC患者血清中前列腺素酶、CA125和LDH水平显著升高。
本研究表明，血清CA125和LDH水平在晚期升高，而前列腺素酶水平在晚期降低。目前的结果表明，与良性及健康对照人群相比，前列腺素酶、CA125和LDH在EOC中存在差异表达，这可能表明其具有更好的诊断价值，具有更高的敏感性和特异性。作者在此采用了一种多标志物方法，包括CA125、AFP、β-hCG、前列腺素酶和LDH，可为EOC患者的鉴别诊断提供更准确的工具。
