Predoi Daniela, Groza Andreea, Iconaru Simona Liliana, Predoi Gabriel, Barbuceanu Florica, Guegan Regis, Motelica-Heino Mikael Stefan, Cimpeanu Carmen
National Institute of Materials Physics, Atomistilor Street, No. 405A, P.O. Box MG 07, 077125 Magurele, Romania.
National Institute for Laser, Plasma and Radiation Physics, 409 Atomistilor Street, P.O. Box MG 36, 077125 Magurele, Romania.
Materials (Basel). 2018 Apr 24;11(5):652. doi: 10.3390/ma11050652.
The research conducted in this study presented for the first time results of physico-chemical properties and in vitro antimicrobial activity of hydroxyapatite plant essential oil against Gram-positive bacteria (methicillin-resistant (MRSA) and 0364) and Gram-negative bacteria ( ATCC 25922). The samples were studied by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy to determine the morphology and structure of the nanocomposites of hydroxyapatite coated with basil (HAp-B) and lavender (HAp-L) essential oils (EOs). The values of the BET specific surface area (S), total pore volume (V) and pore size (D) were determined. The results for the physico-chemical properties of HAp-L and HAp-B revealed that lavender EOs were well adsorbed on the surface of hydroxyapatite, whereas basil EOs showed a poor adsorption on the surface of hydroxyapatite. We found that the lavender EOs hydroxyapatite (HAp-L) exhibited a very good inhibitory growth activity. The value of the minimum inhibitory concentration (MIC) related to growth bacteria was 0.039 mg/mL for MRSA, 0.02 mg/mL for and 0.039 mg/mL ATCC 25922. The basil EO hydroxyapatite (HAp-B) showed poor inhibition of bacterial cell growth. The MIC value was 0.625 mg/mL for the HAp-B sample in the presence of the MRSA bacteria, 0.313 mg/mL in the presence of and 0.078 mg/mL for ATCC 25922.
本研究首次展示了羟基磷灰石植物精油对革兰氏阳性菌（耐甲氧西林金黄色葡萄球菌（MRSA）和0364）以及革兰氏阴性菌（ATCC 25922）的物理化学性质和体外抗菌活性的研究结果。通过扫描电子显微镜（SEM）和傅里叶变换红外（FTIR）光谱对样品进行研究，以确定涂有罗勒（HAp - B）和薰衣草（HAp - L）精油（EOs）的羟基磷灰石纳米复合材料的形态和结构。测定了BET比表面积（S）、总孔体积（V）和孔径（D）的值。HAp - L和HAp - B的物理化学性质结果表明，薰衣草精油能很好地吸附在羟基磷灰石表面，而罗勒精油在羟基磷灰石表面的吸附较差。我们发现薰衣草精油羟基磷灰石（HAp - L）表现出非常好的抑菌生长活性。与生长细菌相关的最低抑菌浓度（MIC）值，对MRSA为0.039 mg/mL，对0364为0.02 mg/mL，对ATCC 25922为0.039 mg/mL。罗勒精油羟基磷灰石（HAp - B）对细菌细胞生长的抑制作用较差。在存在MRSA细菌的情况下，HAp - B样品的MIC值为0.625 mg/mL，在存在0364的情况下为0.313 mg/mL，对ATCC 25922为0.078 mg/mL。
