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锌掺杂羟基磷灰石涂层的结构表征与抗真菌研究

Structural Characterization and Antifungal Studies of Zinc-Doped Hydroxyapatite Coatings.

作者信息

Iconaru Simona Liliana, Prodan Alina Mihaela, Buton Nicolas, Predoi Daniela

机构信息

National Institute of Materials Physics, Atomistilor Street, No. 105 bis, P.O. Box MG 07, 077125 Magurele, Romania.

Emergency Hospital Floreasca Bucharest, 8 Calea Floresca, Sector 1, 014461 Bucharest, Romania.

出版信息

Molecules. 2017 Apr 9;22(4):604. doi: 10.3390/molecules22040604.

Abstract

The present study is focused on the synthesis, characterization and antifungal evaluation of zinc-doped hydroxyapatite (Zn:HAp) coatings. The Zn:HAp coatings were deposited on a pure Si (Zn:HAp_Si) and Ti (Zn:HAp_Ti) substrate by a sol-gel dip coating method using a zinc-doped hydroxyapatite nanogel. The nature of the crystal phase was determined by X-ray diffraction (XRD). The crystalline phase of the prepared Zn:HAp composite was assigned to hexagonal hydroxyapatite in the P6 space group. The colloidal properties of the resulting Zn:HAp (x = 0.1) nanogel were analyzed by Dynamic Light Scattering (DLS) and zeta potential. Scanning Electron Microscopy (SEM) was used to investigate the morphology of the zinc-doped hydroxyapatite (Zn:HAp) nanogel composite and Zn:HAp coatings. The elements Ca, P, O and Zn were found in the Zn:HAp composite. According to the EDX results, the degree of Zn substitution in the structure of Zn:HAp composite was 1.67 wt%. Moreover, the antifungal activity of Zn:HAp_Si and Zn:HAp_Ti against () was evaluated. A decrease in the number of surviving cells was not observed under dark conditions, whereas under daylight and UV light illumination a major decrease in the number of surviving cells was observed.

摘要

本研究聚焦于锌掺杂羟基磷灰石(Zn:HAp)涂层的合成、表征及抗真菌性能评估。采用溶胶 - 凝胶浸涂法,使用锌掺杂羟基磷灰石纳米凝胶,在纯硅(Zn:HAp_Si)和钛(Zn:HAp_Ti)基底上沉积Zn:HAp涂层。通过X射线衍射(XRD)确定晶相性质。制备的Zn:HAp复合材料的晶相归属于P6空间群的六方羟基磷灰石。通过动态光散射(DLS)和zeta电位分析所得Zn:HAp(x = 0.1)纳米凝胶的胶体性质。使用扫描电子显微镜(SEM)研究锌掺杂羟基磷灰石(Zn:HAp)纳米凝胶复合材料和Zn:HAp涂层的形态。在Zn:HAp复合材料中发现了钙(Ca)、磷(P)、氧(O)和锌(Zn)元素。根据能谱分析(EDX)结果,Zn:HAp复合材料结构中锌的取代程度为1.67 wt%。此外,评估了Zn:HAp_Si和Zn:HAp_Ti对()的抗真菌活性。在黑暗条件下未观察到存活细胞数量减少,而在日光和紫外光照射下观察到存活细胞数量大幅减少。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6eb/6154345/2ddde36c3a9b/molecules-22-00604-g001.jpg

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