Dalli Mohammed, El Guerraf Abdelqader, Azizi Salah-Eddine, Benataya Karim, Azghar Ali, Mi-Kyung Jeong, Maleb Adil, Bonglee Kim, Gseyra Nadia
Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, University Mohammed the First, P.O. Box 524, Oujda 60000, Morocco.
Laboratory of Applied Chemistry and Environment, Faculty of Sciences, University Mohammed the First, P.O. Box 524, Oujda 60000, Morocco.
Nanomaterials (Basel). 2022 Mar 3;12(5):856. doi: 10.3390/nano12050856.
As a result of their close similarities to the inorganic mineral components of human bone, hydroxyapatite nanoparticles (n-HAp) are widely used in biomedical applications and for the elaboration of biocompatible scaffold drug delivery systems for bone tissue engineering. In this context, a new efficient and economic procedure was used for the consolidation of n-HAp in the presence of various () fractions at a near-room temperature. The research conducted in the present study focuses on the physicochemical properties of loaded n-HAp 3D scaffolds by fractions and the in vitro antibacterial activity against Gram-negative ( ATCC 25922, ATCC 27853, ATCC 27853), and Gram-positive ( ATCC 29213, ATCC 700603) bacteria. In order to better understand the effect of the inserted fractions on the HAp molecular structure, the elaborated samples were subject to Fourier transform infrared (FTIR) and X-ray diffraction (XRD) spectroscopic analyses. In addition, the morphological investigation by scanning electron microscope (SEM) of the loaded n-HAp 3D scaffolds demonstrated the presence of a porous structure, which is generally required in stimulating bone regeneration. Furthermore, the fabricated 3D composites exhibited significant antibacterial activity against all tested bacteria. Indeed, MIC values ranging from 5 mg/mL to 20 mg/mL were found for the HAp-Ethanol fraction (HAp-Et) and HAp-Hexane fraction (HAp-Hex), while the HAp-Aqueous fraction (HAp-Aq) and HAp-Methanol fraction (HAp-Me) showed values between 20 mg/mL and 30 mg/mL on the different strains. These results suggest that the HAp- scaffolds were effective as a drug delivery system and have very promising applications in bone tissue engineering.
由于羟基磷灰石纳米颗粒（n-HAp）与人体骨骼的无机矿物质成分极为相似，因此被广泛应用于生物医学领域，并用于制备用于骨组织工程的生物相容性支架药物递送系统。在此背景下，一种新的高效且经济的方法被用于在接近室温的条件下，在各种（）组分存在的情况下对n-HAp进行固结。本研究开展的研究聚焦于负载n-HAp三维支架在各组分作用下的物理化学性质，以及对革兰氏阴性菌（ATCC 25922、ATCC 27853、ATCC 27853）和革兰氏阳性菌（ATCC 29213、ATCC 700603）的体外抗菌活性。为了更好地理解插入组分对HAp分子结构的影响，对制备的样品进行了傅里叶变换红外（FTIR）和X射线衍射（XRD）光谱分析。此外，通过扫描电子显微镜（SEM）对负载n-HAp三维支架进行形态学研究，结果表明存在多孔结构，这通常是刺激骨再生所必需的。此外，制备的三维复合材料对所有测试细菌均表现出显著的抗菌活性。事实上，HAp-乙醇组分（HAp-Et）和HAp-己烷组分（HAp-Hex）的最低抑菌浓度（MIC）值在5 mg/mL至20 mg/mL之间，而HAp-水相组分（HAp-Aq）和HAp-甲醇组分（HAp-Me）在不同菌株上的值在20 mg/mL至30 mg/mL之间。这些结果表明，HAp-支架作为药物递送系统是有效的，并且在骨组织工程中具有非常广阔的应用前景。
